Mechanisms of estrogen receptor action in the myocardium. Rapid gene activation via the ERK1/2 pathway and serum response elements

The Journal of Biological Chemistry
T de JagerL Neyses

Abstract

We have previously shown that the myocardium is a target tissue for estrogen. Here, we have identified rapid non-nuclear estrogen effects on the expression of the early growth response gene-1 (Egr-1) in cardiomyocytes. Egr-1 mRNA and protein were rapidly and strongly induced by estrogen in an estrogen receptor-dependent manner via the extracellular signal-regulated kinase, ERK1/2. A promoter analysis study of a 1.2-kilobase Egr-1 promoter fragment revealed that the serum response elements (SREs) but not the estrogen response elements or AP-1 sites are responsible for Egr-1 induction by estrogen, identifying a novel mechanism of estrogen receptor-dependent gene activation in the myocardium. Both estrogen receptor-alpha and -beta induced the Egr-1 promoter via the SREs as well as an artificial promoter consisting of only five SREs in cardiomyocytes. Electrophoretic mobility shift assays showed that a protein complex containing serum response factor or an antigenically related protein was recruited to the SREs by estrogen treatment of primary cardiomyocytes. The recruitment of the protein complex was inhibited by the specific estrogen receptor antagonist ICI 182,780 as well as the MEK inhibitor PD 98059. Taken together, these resu...Continue Reading

References

Aug 1, 1978·Annals of Internal Medicine·T GordonP M McNamara
Nov 1, 1995·Progress in Cardiovascular Diseases·F Grodstein, M Stampfer
Jan 1, 1995·Progress in Nucleic Acid Research and Molecular Biology·A Gashler, V P Sukhatme
Dec 18, 1995·FEBS Letters·R H KarasM E Mendelsohn
May 24, 1996·The Journal of Biological Chemistry·J P AumaisJ H White
Jun 9, 1997·Biochemical and Biophysical Research Communications·H EndohH Kawashima
Jan 8, 1999·Molecular and Cellular Biochemistry·M A PrattD M Novosad
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·T Improta-BrearsD P McDonnell
Jun 11, 1999·The New England Journal of Medicine·M E Mendelsohn, R H Karas
Mar 11, 2000·The Journal of Clinical Investigation·T A McCaffreyT Collins
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·K S RussellJ R Bender

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Citations

May 5, 2006·Nature Reviews. Drug Discovery·Vera Regitz-Zagrosek
Jun 6, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Hajime WatanabeTaisen Iguchi
Feb 16, 2005·Annual Review of Physiology·Dean P Edwards
Jun 5, 2003·The Journal of Clinical Investigation·Stavroula KousteniStavros C Manolagas
Jan 30, 2004·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Reymundo DominguezSonsoles de Lacalle
Sep 27, 2014·Journal of Molecular Endocrinology·Alina GajewskaLech Zwierzchowski
Feb 9, 2016·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Dharmaraj JeyanthiDuraisamy Chellappa
Mar 26, 2013·Toxicology and Applied Pharmacology·Shruti SharanPartha Roy
Jan 30, 2004·International Journal of Cancer. Journal International Du Cancer·Eulalia Pozo-GuisadoPedro M Fernández-Salguero
Jun 15, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Lei LiHong-Wei Li
May 9, 2009·Biochemical and Biophysical Research Communications·Virginija JazbutyteTheo Pelzer
Aug 16, 2005·Progress in Neurobiology·Clive R Bramham, Elhoucine Messaoudi
Nov 8, 2002·Biochimica Et Biophysica Acta·Brian J HarveyChristina M Doolan
Jan 16, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Nayan RoyT Sanjoy Singh
Feb 1, 2015·Frontiers in Neuroendocrinology·Amanda A Krentzel, Luke Remage-Healey
Jun 7, 2014·PloS One·Ming-juan DuYoujia Cao
Dec 3, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·T Sanjoy SinghHarun A R Pramanik
Jan 26, 2017·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Elke Dworatzek, Shokoufeh Mahmoodzadeh
Oct 18, 2003·Journal of Lipid Research·Stefania Lamon-Fava, Dale Micherone
Dec 7, 2007·Circulation·John P Konhilas, Leslie A Leinwand
Nov 2, 2002·Circulation Research·Orlando F Bueno, Jeffery D Molkentin
Dec 17, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Karen J Ho, James K Liao
Feb 8, 2005·Molecular Endocrinology·Linda Björnström, Maria Sjöberg
Oct 27, 2006·The Journal of Biological Chemistry·Jiyuan KeSubbarao Bondada
Sep 18, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Maryanne SkavdahlElizabeth Murphy
Dec 26, 2001·The Journal of Biological Chemistry·Zhiyong GuoFrank Wunderlich
Nov 4, 2016·Physiological Reviews·Vera Regitz-Zagrosek, Georgios Kararigas
Oct 6, 2007·Endocrine Reviews·Stephen R Hammes, Ellis R Levin
Feb 15, 2002·Endocrine Reviews·Johannes PfeilschifterHelmut Schatz

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