Mechanisms of Fibrinogen Adsorption on Silica Sensors at Various pHs: Experiments and Theoretical Modeling

Langmuir : the ACS Journal of Surfaces and Colloids
M WasilewskaMichał Cieśla

Abstract

The adsorption kinetics of human serum fibrinogen at silica substrates was studied using optical waveguide lightmode spectroscopy (OWLS) and quartz crystal microbalance (QCM) techniques. Measurements were performed at pH 3.5, 4, and 7.4 for various ionic strengths. The experimental data were interpreted in terms of a hybrid random sequential adsorption model. This allowed the mass transfer rate coefficient for the OWLS cell and maximum coverages to be determined at various pHs. The appearance of different, pH-dependent mechanisms of fibrinogen adsorption on silica substrates was confirmed. At pH 3.5 the molecules mostly adsorb in the side-on orientation that produces a low maximum coverage of ca. 1 mg m-2. At this pH, the kinetics derived from the OWLS measurements agree with those theoretically predicted using the convective-diffusion theory. In consequence, a comparison of the OWLS and QCM results allows the water factor and the dynamic hydration of fibrinogen molecules to be determined. At pH 7.4, the OWLS method gives inaccurate kinetic data for the low coverage range. However, the maximum coverage that was equal to ca. 4 mg m-2 agrees with the QCM results and with previous literature results. It is postulated that the limi...Continue Reading

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Citations

Jan 14, 2021·Nanomaterials·Anna Bratek-SkickiZbigniew Adamczyk
Jun 3, 2021·International Journal of Environmental Research and Public Health·Monika WasilewskaZbigniew Adamczyk
Jun 8, 2021·Current Opinion in Colloid & Interface Science·Zbigniew AdamczykJakub Barbasz

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