PMID: 11328762May 1, 2001Paper

Mechanisms of fluconazole resistance in Candida albicans isolates from Japanese AIDS patients

The Journal of Antimicrobial Chemotherapy
Kazunori MaebashiHideyo Yamaguchi

Abstract

Four Candida albicans isolates, TIMM 3163, TIMM 3164, TIMM 3165 and TIMM 3166, with reduced fluconazole susceptibility were obtained from three AIDS patients in Japan, and the mechanisms of their drug resistance were studied. All isolates showed lower levels of intracellular accumulation of fluconazole than ATCC 10231, a susceptible control strain of C. albicans. Increased amounts of CDR1 and CDR2 mRNA encoding putative ATP binding cassette (ABC) transporters were associated with the azole resistance of all TIMM isolates, apart from TIMM 3164. In addition, increased Cdr1p levels were immunodetected in the cell membrane fractions of all the TIMM strains except for TIMM 3164. Gene amplification was not responsible for CDR1 overexpression and there were no significant differences in the mRNA levels of CDR3 or CDR4 (ABC transporters) in the azole-susceptible and -resistant cells. CaMDR1 (a major facilitator superfamily) gene expression was not observed in any of the resistant isolates or the control strain. These results suggest that energy-dependent drug efflux associated with increased expression of CDR1 and CDR2 is involved in the fluconazole resistance mechanisms in two of the four isolates, TIMM 3165 and TIMM 3166. TIMM 3164 d...Continue Reading

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Citations

Jan 14, 2003·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Po-Ren HsuehKwen-Tay Luh
Feb 19, 2009·Antimicrobial Agents and Chemotherapy·Sarah TsaoMartine Raymond
Jul 25, 2003·Antimicrobial Agents and Chemotherapy·Emmanuelle PinjonDerek J Sullivan
Apr 16, 2009·Clinical Microbiology Reviews·Richard D CannonBrian C Monk
Dec 8, 2004·Journal of Clinical Microbiology·Preeti PancholiPhyllis Della-Latta
Nov 7, 2006·Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology·Ann R HolmesRichard D Cannon
May 12, 2009·Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology·Kyoko Niimi, Masakazu Niimi
May 15, 2010·Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology·Masakazu Niimi
Jul 15, 2006·Diagnostic Microbiology and Infectious Disease·Mariceli Araujo Ribeiro, Claudete Rodrigues Paula
Oct 28, 2009·Fungal Genetics and Biology : FG & B·Erwin LampingRichard D Cannon
Jun 14, 2008·PLoS Pathogens·Bernardo Ramírez-ZavalaJoachim Morschhäuser
Jun 27, 2009·PLoS Pathogens·Jeffrey J Coleman, Eleftherios Mylonakis
Jun 17, 2010·PloS One·Anatoliy KravetsElena Rustchenko
Jul 28, 2016·Future Medicinal Chemistry·Ann R HolmesRichard D Cannon
Apr 26, 2011·Fungal Genetics and Biology : FG & B·Ting-Li HanSilas G Villas-Bôas
Sep 19, 2001·Yeast
Oct 16, 2004·The Journal of Antimicrobial Chemotherapy·Masakazu NiimiRichard D Cannon

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