Mechanisms of immunity in post-exposure vaccination against Ebola virus infection

PloS One
Steven BradfuteSina Bavari

Abstract

Ebolaviruses can cause severe hemorrhagic fever that is characterized by rapid viral replication, coagulopathy, inflammation, and high lethality rates. Although there is no clinically proven vaccine or treatment for Ebola virus infection, a virus-like particle (VLP) vaccine is effective in mice, guinea pigs, and non-human primates when given pre-infection. In this work, we report that VLPs protect Ebola virus-infected mice when given 24 hours post-infection. Analysis of cytokine expression in serum revealed a decrease in pro-inflammatory cytokine and chemokine levels in mice given VLPs post-exposure compared to infected, untreated mice. Using knockout mice, we show that VLP-mediated post-exposure protection requires perforin, B cells, macrophages, conventional dendritic cells (cDCs), and either CD4+ or CD8+ T cells. Protection was Ebola virus-specific, as marburgvirus VLPs did not protect Ebola virus-infected mice. Increased antibody production in VLP-treated mice correlated with protection, and macrophages were required for this increased production. However, NK cells, IFN-gamma, and TNF-alpha were not required for post-exposure-mediated protection. These data suggest that a non-replicating Ebola virus vaccine can provide post...Continue Reading

References

Sep 5, 1998·The Journal of Infectious Diseases·M BrayJ Huggins
Feb 13, 1999·The Journal of Infectious Diseases·T G KsiazekC J Peters
Feb 13, 1999·The Journal of Infectious Diseases·E I RyabchikovaS V Luchko
Feb 13, 1999·The Journal of Infectious Diseases·B M ConnollyP B Jahrling
Feb 13, 1999·The Journal of Infectious Diseases·N M Kudoyarova-ZubavicheneS V Netesov
Jul 24, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·W ZhongD L Woodland
Jul 27, 2001·Clinical and Experimental Immunology·E M LeroyE Mavoungou
Jan 19, 2002·Journal of Comparative Pathology·T R GibbN K Jaax
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·In-Jeong KimMarcia A Blackman
Feb 27, 2003·Journal of Liposome Research·Nico van Rooijen, Esther van Kesteren-Hendrikx
Dec 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Kelly L WarfieldSina Bavari
Jul 14, 2004·The Journal of Experimental Medicine·Kelly L WarfieldSina Bavari
Sep 18, 2004·Clinical and Experimental Immunology·V Appay
Jan 5, 2005·The Journal of Clinical Investigation·Jeremy S DuffieldJohn P Iredale
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kelly L WarfieldSina Bavari
Oct 28, 2005·The American Journal of Pathology·Jeremy S DuffieldJeremy Hughes
Jan 24, 2007·PLoS Pathogens·Heinz FeldmannThomas W Geisbert
May 17, 2007·European Journal of Immunology·Antony P B BlackGraham S Ogg
Dec 6, 2007·The Journal of Infectious Diseases·Clayton O OnyangoPierre E Rollin
Dec 6, 2007·The Journal of Infectious Diseases·Steven M JonesHeinz Feldmann
Dec 6, 2007·The Journal of Infectious Diseases·Kelly L WarfieldSina Bavari
Oct 24, 2007·Nature Immunology·Carisa A ZampieriGary J Nabel
Mar 7, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Steven B BradfuteSina Bavari
Apr 9, 2008·Expert Review of Vaccines·Chinglai YangRichard W Compans
Jul 22, 2008·Archives of Virology. Supplementum·Jens H Kuhn
Mar 21, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sammy BedouiElodie Segura

❮ Previous
Next ❯

Citations

Oct 2, 2015·Viruses·Scott M Anthony, Steven B Bradfute
Oct 30, 2015·Viruses·Sandra L Bixler, Arthur J Goff
May 11, 2019·The American Journal of Tropical Medicine and Hygiene·Adinarayana KunamneniRavi Durvasula
Mar 5, 2020·Expert Review of Vaccines·Kyle O'Donnell, Andrea Marzi
Sep 2, 2017·Frontiers in Microbiology·Chih-Yun LaiSaguna Verma

❮ Previous
Next ❯

Methods Mentioned

BETA
antisense therapies
ELISAs

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Blood Coagulation Signaling Pathways

Coagulation is the process by which a blood clot is formed. This process includes both the formation of a platelet plug as well as a cascade of clotting factors resulting in the formation of fibrin strands. Find the latest research on coagulation signaling pathways here.

Argentine Hemorrhagic Fever

Argentine hemorrhagic fever (AHF) is an endemo-epidemic disease caused by junín virus (JUNV), a member of the arenaviridae family. Discover the latest research on AHF here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.