Mechanisms of interference of p-diphenols with the Trinder reaction.

Bioorganic Chemistry
Damian TarasekHubert Wojtasek

Abstract

p-Diphenols, such as homogentisic acid, gentisic acid, etamsylate, and calcium dobesilate, interfere with diagnostic tests utilizing the Trinder reaction but the mechanisms of these effects are not fully understood. We observed substantial differences both in oxidation of p-diphenols by horseradish peroxidase and their influence on oxidation of 4-aminoantipyrine and various phenolic substrates. Homogentisic acid was rapidly oxidized by the enzyme and completely blocked chromophore formation. Enzymatic oxidation of the remaining p-diphenols was slow and they only moderately inhibited chromophore formation. However, in the presence of standard substrates all tested p-diphenols were rapidly converted to p-quinones. Hydrogen peroxide consumption was significantly accelerated by homogentisic acid but not much affected by the other p-diphenols. The magnitude and mechanisms of interference caused by p-diphenols therefore depend on their structure which determines their electrochemical properties - while for homogentisic acid with an electron-donating substituent and a lower reduction potential both enzymatic oxidation and reduction of the peroxidase-generated radicals occur, for p-diphenols with electron-withdrawing substituents and h...Continue Reading

References

Aug 1, 1972·Clinica Chimica Acta; International Journal of Clinical Chemistry·P Sharp
Nov 1, 1995·Diabetes Research and Clinical Practice·E NakashimaN Hotta
Jan 29, 1962·Biochimica Et Biophysica Acta·S J KLEBANOFF
Jan 13, 2006·Clinical Biochemistry·Flávia Martinello, Edson Luiz da Silva
Jun 30, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Flávia Martinello, Edson Luiz da Silva
Mar 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Thomas W SedlakSolomon H Snyder
Jul 11, 2009·Clinical Chemistry·Amy K SaengerAllan S Jaffe
Feb 9, 2010·Molecular Genetics and Metabolism·Perry R LokenKimiyo Raymond
Nov 29, 2011·Analytical and Bioanalytical Chemistry·Elise F Hoek-van den HilEvert M van Schothorst
Apr 17, 2012·Clinical Chemistry and Laboratory Medicine : CCLM·Steven PauwelsPieter Vermeersch
Feb 7, 2013·The Journal of Physical Chemistry. B·Marzieh EslamiHamid R Zare
May 22, 2013·Scandinavian Journal of Clinical and Laboratory Investigation·Ondrej WiewiorkaZdenka Čermáková
Oct 27, 2015·Clinical Biochemistry·Jonathan R GenzenMatthew D Krasowski
Dec 15, 2016·Journal of the American Chemical Society·Mioy T HuynhSharon Hammes-Schiffer
Sep 5, 2017·Bioorganic & Medicinal Chemistry Letters·J Michael Ellis, Matthew J Fell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved