Mechanisms of killing of newborn larvae of Trichinella spiralis by neutrophils and eosinophils. Killing by generators of hydrogen peroxide in vitro

The Journal of Clinical Investigation
D A Bass, P Szejda

Abstract

Eosinophil and/or neutrophil leukocytes appear to have important roles in host defense against invasive, migratory helminth infestations, but the mechanisms of larval killing by leukocytes are uncertain. This study examines killing of newborn (migratory phase) larvae of Trichinella spiralis during incubation with granule preparations of human eosinophils or neutrophils and generators of hydrogen peroxide (glucose-glucose oxidase) (G-GO) or superoxide and hydrogen peroxide (xanthine-xanthine oxidase). Larvae were killed by either hydrogen peroxide-generating system in a concentration-dependent manner. Direct enumeration of surviving larvae after incubation in microtiter wells containing the appropriate reagents was used in assess larval killing. Verification of the microplate assay was demonstrated by complete loss of larval ability to incorporate [(3)H]deoxyglucose and loss of infectivity after incubation in comparable concentrations of G-GO. Larvae were highly sensitive to oxidative products; significant killing occurred after incubation with 0.12 mU glucose oxidase and complete killing occurred with 0.5 mU. Comparable killing of bacteria required over 60 mU glucose oxidase. At 5 mU glucose oxidase, killing was complete after ...Continue Reading

References

Feb 1, 1978·Biochemical Medicine·R Migler, L R DeChatelet
Jun 1, 1975·The Journal of Experimental Medicine·R A Clark, S J Klebanoff
Sep 1, 1976·The Journal of Cell Biology·R A ClarkS J Klebanoff
Jun 1, 1977·Proceedings of the Society for Experimental Biology and Medicine·G IsmailW S Wegener
Mar 1, 1977·The Journal of Experimental Medicine·D I GroveK S Warren
Jan 1, 1978·The Journal of Experimental Medicine·F J Ramalho-PintoS R Smithers
Dec 21, 1978·Nature·C D MackenzieB M Ogilvie
Jul 1, 1973·The Journal of Experimental Medicine·P J Edelson, Z A Cohn
Dec 1, 1967·The Journal of Experimental Medicine·S J Klebanoff
Jun 1, 1970·The Journal of Experimental Medicine·A Basten, P B Beeson
Nov 21, 1964·Nature·A BOYUM

Citations

May 23, 2009·Parasitology Research·Lorena Chiumiento, Fabrizio Bruschi
Apr 1, 1989·International Journal for Parasitology·T L Rothwell
Oct 1, 1981·Molecular and Biochemical Parasitology·H W MurrayW A Scott
Jan 1, 1984·Molecular and Biochemical Parasitology·J W Kazura, S R Meshnick
Jul 1, 1986·Parasitology Today·T D LeeA D Befus
Aug 1, 1988·Parasitology Today·H L CallahanE R James
Dec 4, 2001·International Journal for Parasitology·A C Kotze, S J McClure
May 30, 2001·Molecular and Biochemical Parasitology·K Henkle-Dührsen, A Kampkötter
Jan 18, 2002·Postgraduate Medical Journal·Fabrizio Bruschi, K D Murrell
Sep 1, 1981·The Journal of Clinical Investigation·S J WeissN F Nimeh
Apr 1, 1985·The Journal of Clinical Investigation·J W KazuraM Aikawa
Sep 1, 1984·Veterinary Parasitology·K D Murrell, D D Despommier
Jan 1, 1988·Parasite Immunology·R M CookJ Chernin
Nov 15, 1990·Biochemical Pharmacology·S BatraV M Srivastava
Jul 24, 2015·Acta Parasitologica·Klaudia BrodaczewskaMaria Doligalska
Aug 30, 2008·Trends in Parasitology·Fabrizio BruschiNaohiro Watanabe
Jan 1, 1983·Journal of Cellular Biochemistry·R M Locksley, S J Klebanoff
Aug 5, 2016·PLoS Neglected Tropical Diseases·Gwendoline DeslyperJames C Carolan
Oct 1, 1993·Parasite Immunology·S M VenturielloS N Costantino
Apr 1, 1992·Annals of Tropical Medicine and Parasitology·N W Brattig, K D Erttmann

Related Concepts

Blood Bactericidal Activity
Eosinophil
Glucose Oxidase
Oxydol
Hydrogen-Ion Concentration
Neutrophil Band Cells
Ovoperoxidase
Superoxides
Human Trichinellosis
Xanthine Oxidase

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.