Mechanisms of nitric-oxide-induced increase of free cytosolic Ca2+ concentration in Nicotiana plumbaginifolia cells

Free Radical Biology & Medicine
Olivier LamotteDavid Wendehenne

Abstract

In this study, we investigated a role for nitric oxide (NO) in mediating the elevation of the free cytosolic Ca(2+) concentration ([Ca(2+)](cyt)) in plants using Nicotiana plumbaginifolia cells expressing the Ca(2+) reporter apoaequorin. Hyperosmotic stress induced a fast increase of [Ca(2+)](cyt) which was strongly reduced by pretreating cell suspensions with the NO scavenger carboxy PTIO, indicating that NO mediates [Ca(2+)](cyt) changes in plant cells challenged by abiotic stress. Accordingly, treatment of transgenic N. plumbaginifolia cells with the NO donor diethylamine NONOate was followed by a transient increase of [Ca(2+)](cyt) sensitive to plasma membrane Ca(2+) channel inhibitors and antagonist of cyclic ADP ribose. We provided evidence that NO might activate plasma membrane Ca(2+) channels by inducing a rapid and transient plasma membrane depolarization. Furthermore, NO-induced elevation of [Ca(2+)](cyt) was suppressed by the kinase inhibitor staurosporine, suggesting that NO enhances [Ca(2+)](cyt) by promoting phosphorylation-dependent events. This result was further supported by the demonstration that the NO donor induced the activation of a 42-kDa protein kinase which belongs to SnRK2 families and corresponds to N...Continue Reading

References

Feb 16, 1996·The Journal of Biological Chemistry·N WillmottA Galione
Apr 1, 1996·Journal of Photochemistry and Photobiology. B, Biology·J Plásek, K Sigler
Jul 1, 1997·Neuroscience·J S Bains, A V Ferguson
Apr 4, 1998·Brain Research. Molecular Brain Research·S OhkumaK Kuriyama
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·J DurnerD F Klessig
Nov 10, 1998·The Plant Journal : for Cell and Molecular Biology·A Lebrun-GarciaA Pugin
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·C P LeckieA M Hetherington
Apr 5, 2000·Biochimica Et Biophysica Acta·P J White
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·D F KlessigH Silva
Mar 23, 2002·Free Radical Biology & Medicine·Jianguo ChenToshinori Hoshi
Dec 18, 2002·Archives of Biochemistry and Biophysics·Joan B Mannick, Christopher M Schonhoff
Sep 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Carlos Garcia-MataMichael R Blatt
Dec 4, 2003·Molecular Plant-microbe Interactions : MPMI·Annalisa PolverariMassimo Delledonne
Jul 3, 2004·Current Opinion in Plant Biology·David WendehenneDaniel F Klessig
Mar 1, 2005·Plant Physiology·Christian LindermayrJörg Durner

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Citations

Apr 16, 2013·Plant Cell Reports·Marcela SimontacchiLorenzo Lamattina
Nov 26, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wei MaGerald A Berkowitz
Nov 26, 2010·The Journal of Biological Chemistry·Maria BucholcGrażyna Dobrowolska
Dec 6, 2011·Omics : a Journal of Integrative Biology·Anna KulikGrażyna Dobrowolska
Feb 2, 2013·AoB Plants·Luis A J MurKapuganti J Gupta
Jul 15, 2006·Molecular Plant-microbe Interactions : MPMI·Angela Garcia-BruggerAlain Pugin
Aug 14, 2012·Plant Physiology·Anna KulikGrazyna Dobrowolska
Nov 23, 2007·Annual Review of Plant Biology·Angélique Besson-BardDavid Wendehenne
Feb 11, 2014·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Huyi HeMinghua Gu
Aug 14, 2012·Nitric Oxide : Biology and Chemistry·M Nasir KhanM Naeem
Aug 17, 2012·Journal of Environmental Sciences (China)·Long ZhangCheng Zhu
Sep 7, 2011·Plant Science : an International Journal of Experimental Plant Biology·Alla I YemetsYaroslav B Blume
Sep 7, 2011·Plant Science : an International Journal of Experimental Plant Biology·Jéremy AstierDavid Wendehenne
Mar 5, 2011·The New Phytologist·Lyudmila V DubovskayaIgor D Volotovski
Feb 15, 2015·Molecular Plant·Patricia DomingosJose A Feijo
Apr 10, 2014·Phytochemistry·Pauline TrapetDavid Wendehenne
Jun 5, 2010·Molecular Plant·Christian MazarsStéphane Bourque
Mar 1, 2008·Molecular Plant·Angélique Besson-BardDavid Wendehenne
May 13, 2011·Plant Physiology and Biochemistry : PPB·Doriana FranciaFrancesca Cardinale
Oct 10, 2009·Plant Signaling & Behavior·Ian D WilsonJohn T Hancock
Jul 22, 2018·Journal of Integrative Plant Biology·Bingjie LiWenqiang Tang
Oct 1, 2019·Plant Signaling & Behavior·Maryam SeifikalhorTao Li
Sep 2, 2009·Plant Signaling & Behavior·Mourad A M Aboul-SoudGary J Loake
Feb 27, 2017·Environmental Science and Pollution Research International·Min YiHuilan Yi
Sep 21, 2018·Frontiers in Plant Science·Jing ZhangWeibiao Liao

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