Mechanisms of nonhomologous DNA end-joining in frogs, mice and men

Biological Chemistry
P DazaPetra Pfeiffer

Abstract

DNA end-joining, a process related to illegitimate recombination and capable of rejoining unrelated pairs of DNA ends in the absence of sequence homology, is considered the major pathway of double-strand break (DSB) repair in mammalian cells. Whole cell and nuclear extracts from three human and one mouse cell line were investigated for their capacities to promote nonhomologous DNA end-joining and their relative activities of DNA-PK, a mammalian DNA end-binding protein complex implicated in DSB-repair. The levels of DNA end-joining and the spectra of junctions of the human systems were identical with the ones of a previously described cell-free joining system derived from Xenopus laevis eggs. Due to the presence of potent 3'-5'-exonuclease activities the mouse system displayed decreased levels of DNA end-joining and larger fractions of junctions containing deletions but otherwise the basic mechanisms of junction formation appeared to be identical with the Xenopus system. DNA-PK activity was found to be equally low in the Xenopus and the mouse system but 4- to 6-fold increased in the human systems. Our results suggest that the mechanisms of DNA end-joining may be modulated by the level of exonuclease activities and/or DNA end-pro...Continue Reading

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Citations

Nov 20, 1998·Toxicology Letters·P Pfeiffer
Jan 2, 2003·DNA Repair·Thierry Nouspikel, Philip C Hanawalt
Jan 2, 2003·DNA Repair·Ana Sandoval, Paul Labhart
Oct 12, 2010·Journal of Nucleic Acids·Sheetal Sharma, Sathees C Raghavan
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Mar 19, 1999·Molecular and Cellular Biology·P Labhart
Oct 16, 1999·European Journal of Biochemistry·P Labhart

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