PMID: 8961380Jan 1, 1996Paper

Mechanisms of oxyradical production in substance P stimulated rheumatoid synovial cells

Rheumatology International
T TanabeC Inagaki

Abstract

We examined the intracellular mechanisms of substance P induced oxyradical production in rheumatoid synovial cells by the luminol-dependent chemiluminescence method. After stimulation with substance P (30 microM), single synovial A (macrophage-like) or B (fibroblast-like) cells released oxyradicals such as superoxide anions (O2-) and/or hypochlorous anions (OCl-) under a microscope equipped with an ultrasensitive photonic image intensifier. The substance P induced oxyradical production was blocked by a tachykinin NK1 (NK1) receptor antagonist, GR82334, GTP-binding protein (G-protein) inactivators, GDP beta S and islet-activating protein (IAP), and a phospholipase C (PLC) inhibitor, U-73122. Substance P (30 microM) also induced a transient increase in the intracellular Ca2+ concentration ([Ca2+]i) in both synovial A and B cells as measured by a Ca2+ indicator, fura 2, BAPTA-AM and an inositol-1,4-5-triphosphate (IP3) receptor antagonist, heparin, inhibited the substance P induced increase in [Ca2+]i, but they had no effects on oxyradical production. In contrast to the effects of BAPTA-AM and heparin, protein kinase C (PKC) inhibitors, H-7 and calphostin C, completely inhibited substance P induced oxyradical production without an...Continue Reading

References

Mar 8, 1976·Biochemical and Biophysical Research Communications·R C Allen, L D Loose
Jan 1, 1990·Annals of the New York Academy of Sciences·E S Kimball
Jan 1, 1990·Annals of the New York Academy of Sciences·J P McGillisD G Payan
May 1, 1991·The American Journal of Physiology·S Grinstein, W Furuya
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T Meyer, L Stryer
Mar 15, 1989·Biochemical and Biophysical Research Communications·E KobayashiT Tamaoki
Feb 1, 1989·Journal of Applied Physiology·C GattoD E Johnson
Jan 1, 1989·Springer Seminars in Immunopathology·C T Ritchlin, R J Winchester
Dec 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P M LadS J Scott
Dec 31, 1987·Biochemical and Biophysical Research Communications·M SuematsuM Tsuchiya
Nov 1, 1982·Arthritis and Rheumatism·E M TanR J Winchester
Jun 1, 1994·Arthritis and Rheumatism·N J Zvaifler, G S Firestein
Mar 28, 1996·European Journal of Pharmacology·T TanabeC Inagaki

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Citations

Apr 28, 2001·Japanese Journal of Pharmacology·K MishimaC Inagaki
Jan 5, 2001·Expert Opinion on Investigational Drugs·S Brunelleschi
Jan 13, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·B OssyssekR H Straub
Jan 11, 2005·Rheumatic Diseases Clinics of North America·Hans-Georg SchaibleMarco Matucci-Cerinic
Apr 30, 2017·International Journal of Molecular Sciences·Susanne Grässel, Dominique Muschter
Sep 29, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Birgit LehnerRainer H Straub
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·L E MillerR H Straub
Apr 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·C Kumaran, K Shivakumar
Mar 7, 2003·American Journal of Physiology. Renal Physiology·Chiang-Ting ChienSu-Ming Hsu

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