PMID: 7541950Jun 1, 1995Paper

Mechanisms of pertussis toxin-induced barrier dysfunction in bovine pulmonary artery endothelial cell monolayers

The American Journal of Physiology
C E PattersonJ G Garcia

Abstract

We have previously characterized several G proteins in endothelial cells (EC) as substrates for the ADP-ribosyltransferase activity of both pertussis (PT) and cholera toxin and described the modulation of key EC physiological responses, including gap formation and barrier function, by these toxins. In this study, we investigated the mechanisms involved in PT-mediated regulation of bovine pulmonary artery endothelial cells barrier function. PT caused a dose-dependent increase in albumin transfer, dependent upon action of the holotoxin, since neither the heat-inactivated PT, the isolated oligomer, nor the protomer induced EC permeability. PT-induced gap formation and barrier dysfunction were additive to either thrombin- or thrombin receptor-activating peptide-induced permeability, suggesting that thrombin and PT utilize distinct mechanisms. PT did not result in Ca2+ mobilization or alter either basal or thrombin-induced myosin light chain phosphorylation. However, PT stimulated protein kinase C (PKC) activation, and both PKC downregulation and PKC inhibition attenuated PT-induced permeability, indicating that PKC activity is involved in PT-induced barrier dysfunction. Like thrombin-induced permeability, the PT effect was blocked ...Continue Reading

Citations

Dec 1, 1998·Endothelium : Journal of Endothelial Cell Research·C E PattersonJ G Garcia
May 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·F LiuJ G Garcia
Apr 5, 2001·American Journal of Physiology. Cell Physiology·J G GarciaA D Verin
Mar 11, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kane L SchaphorstJoe G N Garcia
Nov 13, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·P WangJ G Garcia
Jun 19, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·K A MohammedV B Antony
May 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·I PetracheJ G Garcia
May 13, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Natalia V BogatchevaJoe G N Garcia
Dec 24, 2005·Physiological Reviews·Dolly Mehta, Asrar B Malik
Apr 8, 2020·Toxins·Rudolf LucasJürg Hamacher
May 20, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Randal O DullJoe G N Garcia
Dec 26, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sergey I ZharikovEdward R Block

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