Mechanisms of platelet activating factor-induced aggregation and secretion in human platelets

Prostaglandins
R K McCulloch, R Vandongen

Abstract

The role of TXA2 in PAF-induced aggregation and secretion of human platelets is unclear. We have studied the relationship between aggregation, synthesis of TXA2 and release of 5-HT during the time course of aggregation induced by PAF and collagen. For PAF-induced aggregation there was strong aggregation and secretion with minimal production of TXA2 in contrast to collagen in which a surge in TXA2 synthesis preceded both aggregation and secretion. To determine the role of calcium flux in PAF-induced aggregation we have similarly studied the temporal relationships between aggregation, secretion and TXA2 synthesis for calcium ionophore A23187 induced aggregation but found these to be distinctly different from those determined for PAF. A method for measuring absolute amounts of 5HT released from platelets in small volumes of plasma is described. We conclude that TXA2 is not important in the mechanism of PAF induced aggregation and that an increase in the level of intraplatelet calcium per se is not sufficient to explain the mediation of PAF-induced aggregation.

References

Oct 15, 1987·Thrombosis Research·F VioliC Cordova
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Aug 1, 1984·Veterinary Immunology and Immunopathology·H D LiggittL Huston
Nov 1, 1984·British Journal of Pharmacology·F Fouque, B B Vargaftig
Jun 1, 1980·Scientific American·M B Zucker
Mar 1, 1981·The Journal of Clinical Investigation·L M McManusR N Pinckard

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Citations

Jul 16, 2003·Journal of Ethnopharmacology·Rattima JeenapongsaKampon Sriwatanakul
Sep 1, 1992·Thrombosis Research·P V VrzheshchS D Varfolomeyev
Dec 1, 1990·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D J FretlandT S Gaginella

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