PMID: 697767Jul 15, 1978Paper

Mechanisms of replenishment of nuclear androgen receptor in rat ventral prostate

The Biochemical Journal
E Van Doorn, N Bruchovsky

Abstract

1. The concentration of androgen receptor in the nucleus of the prostatic cell is rapidly elevated by the administration in vivo of 2mug of [(3)H]testosterone to 1-day-castrated rats. From a concentration of 2300 receptors/nucleus at 5min after intravenous injection of hormone, there is an increase to 21000 receptors/nucleus at 60min. At the same time, the amount of binding of androgen in the cytoplasm remains constant at a relatively low value. 2. An identical dose of [(3)H]testosterone administered to 7-day-castrated rats produces a much smaller change in the concentration of nuclear receptor, from 700 receptors/nucleus at 5min to only 4300 receptors/nucleus at 60min. Thus the reservoir from which nuclear receptor is replenished is considerably smaller in regressed prostatic cells. Again, the amount of binding of androgen in the cytoplasm remains unchanged at a low value over the experimental time course of 60min. 3. In contrast with the scant labelling of cytoplasmic receptor achieved by injecting animals with [(3)H]testosterone, labelling in vitro, by incubation of tissue slices with radioisotope, indicates that prostate of 1-day-castrated animals actually contains 21400 receptors/cell in the cytoplasmic compartment, and pr...Continue Reading

Citations

Nov 3, 2004·Trends in Endocrinology and Metabolism : TEM·Ben E Black, Bryce M Paschal
Jan 1, 1995·International Urology and Nephrology·V L Kumar, P K Majumder
Nov 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·J V Choate, J A Resko
Sep 27, 1985·Biochimica Et Biophysica Acta·J SteinsapirT G Muldoon
May 1, 1984·Journal of Steroid Biochemistry·C G Pierrepoint, P Davies
Apr 1, 1990·Journal of Steroid Biochemistry·M FuruyamaM Hosaka

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