Mechanisms of resistance of HIV-1 primary isolates to complement-mediated lysis

Virology
D M TakefmanG T Spear

Abstract

Previous studies suggested that HIV-1 primary isolates (PI) were resistant to complement-mediated lysis (CML), while virus produced in certain T cell lines and virus taken directly from the plasma of HIV+ persons were both susceptible to CML. The purpose of this study was to investigate the mechanism(s) of PI resistance. PI were resistant to CML using pooled seropositive serum as an antibody source. Additionally, PI obtained from two patients at several times over 2 years were resistant to CML using autologous antibody. PI were also resistant to CML induced by monoclonal antibodies which neutralize a broad range of PI. Resistance to CML was associated with low binding of antibody to PI but was not due to low gp120 levels. Cell-line-derived virus and PI were equally sensitive to CML induced by antibody to host-cell proteins, suggesting that PBMC do not contribute properties to virions which make them more physically resistant to CML in general but that PI resistance is restricted to CML induced by antiviral antibody. These studies show that PI are resistant to CML mediated by various antiviral antibodies and indicate that low binding of antibody to virus is an important factor contributing to resistance.

References

Sep 1, 1990·The Journal of Infectious Diseases·M Tremblay, M A Wainberg
Mar 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M YoshidaY Hinuma
Jul 1, 1994·AIDS Research and Human Retroviruses·M SaifuddinG T Spear
Jan 1, 1993·Journal of Virology·G T SpearS Zolla-Pazner
Jun 1, 1994·AIDS Research and Human Retroviruses·P Innocenti-FrancillardJ M Seigneurin
May 1, 1996·Immunology Today·P PoignardQ J Sattentau

❮ Previous
Next ❯

Citations

Oct 19, 1999·The Journal of Experimental Medicine·A F OchsenbeinR M Zinkernagel
Dec 19, 2002·AIDS Research and Human Retroviruses·Melanie L HartGregory T Spear
Feb 28, 2002·AIDS Research and Human Retroviruses·Gene G OlingerGregory T Spear
May 1, 2004·AIDS Research and Human Retroviruses·Hongyu YingGregory T Spear
Aug 19, 2008·Virology Journal·Joshua J AnzingerGregory T Spear
Nov 30, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kyung Min ChungMichael S Diamond
Jan 31, 2002·AIDS·F P Siegal, G T Spear
Jan 15, 2008·Vaccine·Heribert StoiberManfred P Dierich
Aug 28, 2003·Immunology·Gregory T SpearMohammed Saifuddin
Jun 30, 2007·Journal of Internal Medicine·M Huber, A Trkola
Oct 19, 2004·Molecular Immunology·Heribert StoiberManfred P Dierich
Jul 18, 2006·Virus Research·Joshua J AnzingerGregory T Spear
Apr 24, 2009·Vaccine·Panisadee AvirutnanMichael S Diamond
Apr 24, 2009·Vaccine·Heribert StoiberManfred P Dierich
Feb 13, 2001·Clinical Immunology : the Official Journal of the Clinical Immunology Society·J R SalkowitzM M Lederman
Mar 22, 2000·The Journal of General Virology·M SaifuddinG T Spear
Sep 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eliska HorakovaJürg A Schifferli

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.