Mechanisms of resistance to imipenem in imipenem-resistant, ampicillin-sensitive Enterococcus faecium

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
N El AminB Wretlind

Abstract

Enterococcus faecium has six penicillin-binding proteins (PBP), where PBP5 seems to be the main target for beta-lactam antibiotics. The PBP profiles of three imipenem-resistant, ampicillin-sensitive E. faecium strains, isolated from the same patient, were studied using biotinylated ampicillin and chemiluminescence detection. Imipenem resistance in these strains was found to be associated with hyperproduction of PBP5 compared to the ampicillin- and imipenem-susceptible strain ATCC 19434. PBP5 in the imipenem-resistant strains (S1, B2) exhibited a selectively decreased affinity for imipenem. An 854 bp DNA fragment, corresponding to the penicillin-binding domain of pbp5fm, was studied in the resistant strains and the reference strain. Four amino acid substitutions were observed in the resistant strains compared to the susceptible one. The contribution of these substitutions to the increased production of PBP5 in these strains is unclear since the substitution was observed also in a strain without increased production of PBP5. Our results suggest that the moderate imipenem resistance observed in these strains is associated with increased production of PBP5 with relatively decreased affinity for imipenem, and that evolution of imipe...Continue Reading

Citations

Sep 29, 2005·Antimicrobial Agents and Chemotherapy·Yutaka UedaMakoto Sunagawa
Feb 5, 2002·Journal of Clinical Microbiology·Nagwa El AminJacques Bille
Aug 22, 2008·International Journal of Antimicrobial Agents·Hanna BillströmCarl Erik Nord
Jun 30, 2015·Future Microbiology·J M PericásJ M Miró
Dec 3, 2014·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Natália ConceiçãoAdriana Gonçalves de Oliveira
Nov 26, 2005·The Journal of Antimicrobial Chemotherapy·Evgenia MetzidieAthanassios Tsakris

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