Mechanisms of sex determination and transmission ratio distortion in Aedes aegypti

Parasites & Vectors
Kim Phuc HoangVinh Sy Le

Abstract

More effective mosquito control strategies are urgently required due to the increasing prevalence of insecticide resistance. The sterile insect technique (SIT) and the release of insects carrying a dominant lethal allele (RIDL) are two proposed methods for environmentally-friendly, species-targeted population control. These methods may be more suitable for developing countries if producers reduce the cost of rearing insects. The cost of control programs could be reduced by producing all-male mosquito populations to circumvent the isolation of females before release without reducing male mating competitiveness caused by transgenes. An RNAi construct targeting the RNA recognition motif of the Aedes aegypti transformer-2 (tra-2) gene does not trigger female-to-male sex conversion as commonly observed among dipterous insects. Instead, homozygous insects show greater mortality among m-chromosome-bearing sperm and mm zygotes, yielding up to 100% males in the subsequent generations. The performance of transgenic males was not significantly different to wild-type males in narrow-cage competitive mating experiments. Our data provide preliminary evidence that the knockdown of Ae. aegypti tra-2 gene expression causes segregation distortio...Continue Reading

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Citations

Dec 6, 2017·BMC Genomics·Corey L CampbellWilliam C Black
Dec 26, 2018·Parasites & Vectors·Helena Rocha Corrêa de AraújoMargareth Lara Capurro
Jan 10, 2017·Insects·Paul M Airs, Lyric C Bartholomay
Feb 23, 2020·Frontiers in Immunology·Rebecca L BelmonteJennifer C Regan

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Datasets Mentioned

BETA
AAEL009222
476766.1
KJ147316
KJ147314
KJ147320
KJ147315
KC710231.1
AJ427611.1
AB236435.1
KJ147319

Methods Mentioned

BETA
transgenic
PCR
dissection

Software Mentioned

MEGA5
DNAsp
Excel
PROSITE
Python

Related Concepts

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