PMID: 6794451Sep 1, 1981Paper

Mechanisms of sorbate inhibition of Bacillus cereus T and Clostridium botulinum 62A spore germination

Applied and Environmental Microbiology
L A Smoot, M D Pierson

Abstract

The mechanism by which potassium sorbate inhibits Bacillus cereus T and Clostridium botulinum 62A spore germination was investigated. Spores of B. cereus T were germinated at 35 degrees C in 0.08 M sodium-potassium phosphate buffers (pH 5.7 and 6.7) containing various germinants (L-alanine, L-alpha-NH2-n-butyric acid, and inosine) and potassium sorbate. Spores of C. botulinum 62A were germinated in the same buffers but with 10 mM L-lactic acid, 20 mM sodium bicarbonate, L-alanine or L-cysteine, and potassium sorbate. Spore germination was monitored by optical density measurements at 600 nm and phase-contrast microscopy. Inhibition of B. cereus T spore germination was observed when 3,900 micrograms of potassium sorbate per ml was added at various time intervals during the first 2 min of spore exposure to the pH 5.7 germination medium. C. botulinum 62A spore germination was inhibited when 5,200 micrograms of potassium sorbate per ml was added during the first 30 min of spore exposure to the pH 5.7 medium. Potassium sorbate inhibition of germination was reversible for both B. cereus T and C. botulinum 62A spores. Potassium sorbate inhibition of B. cereus T spore germination induced by L-alanine and L-alpha-NH2-n-butyric acid was s...Continue Reading

Citations

Mar 12, 2004·Journal of Applied Microbiology·D E CortezzoP Setlow
Aug 9, 2017·Journal of Food Protection·Kathleen A GlassFrank Rossi
Nov 1, 1985·The Journal of Applied Bacteriology·J C Blocher, F F Busta
Jan 1, 1982·Critical Reviews in Food Science and Nutrition·M D Pierson, L A Smoot
Aug 25, 2015·Frontiers in Microbiology·Sara E DiomandéJulien Brillard
Apr 20, 2016·Frontiers in Microbiology·Kevin EganPaul D Cotter
Feb 1, 2011·Applied and Environmental Microbiology·C C J van MelisTjakko Abee

Related Concepts

Alpha-aminobutyric acid, (R)-isomer
Abufne
Aminobutyrates
Bacillus cereus
Clostridium botulinum
Fatty Acids, Unsaturated
Sodium Sorbate
Spores, Bacterial

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