Mechanisms of Tanshinone II a inhibits malignant melanoma development through blocking autophagy signal transduction in A375 cell

BMC Cancer
Xiaojing LiZhijun Liu

Abstract

Malignant melanoma (MM) is one of the high degree of malignancy and early prone to blood and lymph node metastasis. There is not cured for MM. Tan II A has been reported to reduce cancer cell proliferation. But the mechanism by which Tan II A inhibited melanoma growth are not well characterized. We sought to explore the possible mechanism by which Tan II A regulated cell proliferation through autophagy signaling pathway in A375 cells. We tested the effects of Tan II A on melanoma A375, MV3, M14, and other human cell lines including Hacat and HUVEC cells in cell culture model. Cell proliferation was assessed by using methyl thiazol tetrazolium (MTT) assay. Cell migration ability melanoma A375 was monitored by using cell scratch assay. Transwell chamber experimental was performed to assess the effect of Tan II A on A375 melanoma cell invasion ability. The autophagy body was examined by using flow cytometry. The expression of autophagy-associated protein beclin-1 and microtubule-associated protein 1 light chain 3(LC3)-II, as well as phosphatidylinositol 3-kinase(PI3K)、protein kinase B (Akt)、mammalian target of rapamycin (mTOR)、p70S6K1 signaling pathways were detected by using Western blotting. The effects of Tan II A on tumor prog...Continue Reading

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Citations

Sep 28, 2017·International Journal of Molecular Sciences·Yu-Kyoung ParkByeong-Churl Jang
Mar 28, 2018·Experimental and Therapeutic Medicine·Hong Liu, Mingming Cai
Aug 2, 2017·International Journal of Molecular Sciences·Sung-Ying HuangSheng-Chun Chiu

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Methods Mentioned

BETA
flow cytometry
X-ray

Software Mentioned

SPSS17

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