Mechanisms of the anti-inflammatory effects of hydroxyethyl starch demonstrated in a flow-based model of neutrophil recruitment by endothelial cells

Critical Care Medicine
Nick M MatharuGerard B Nash

Abstract

To determine whether plasma volume expander hydroxyethyl starch (HES) may protect against reperfusion injury through an ability to reduce neutrophil recruitment. An in vitro study using paired comparisons of adhesion of flowing neutrophils. A collaboration between clinical and basic science departments in a university hospital. Neutrophils and cultured human umbilical vein endothelial cells (HUVEC). Treatment with HES (average molecular weight of 200 kd and substitution of 0.62) at clinically relevant concentrations or with gelatin solution (average molecular weight of 30 kDa) of comparable viscosity. Glass capillaries were coated internally with either purified adhesion molecules or HUVEC, which were treated with tumor necrosis factor-alpha in the presence or absence of HES. Neutrophils were perfused over these surfaces (with or without HES) and their recruitment quantified by video microscopy. Expression of adhesion molecules and of the chemokine interleukin-8 by HUVEC were analyzed by enzyme-linked immunosorbent assay and quantitation of messenger RNA. HES over a wide range of concentrations had no effect on selectin- or integrin-mediated adhesion of neutrophils. However, when HUVEC were cultured with 1.5% wt/vol HES, neutro...Continue Reading

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May 2, 2008·Critical Care Medicine·Linda A KirschenbaumMark E Astiz
Sep 25, 2008·Critical Care Medicine·Peter Gosling
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Feb 17, 2019·International Journal of Molecular Sciences·Alessandro TrentiniTiziana Bellini

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