Mechanisms of TiO2 NPs-induced phoxim metabolism in silkworm (Bombyx mori) fat body

Pesticide Biochemistry and Physiology
J S HuBing Li

Abstract

Silkworm is an important economic insect. Abuse of organophosphorus pesticides in recent years often leads to poisoning of silkworms, which significantly affects sericulture development by reducing silk production. Previous studies have shown that TiO2 NPs can effectively mitigate the damages caused by organophosphorus pesticides in silk glands and nerve tissues. The fat body is an important metabolic detoxification organ of silkworms, but it is unknown whether TiO2 NPs affect pesticide metabolism in fat body. In this study, we characterized the transcription of antioxidant genes and enzyme activity in fat body after TiO2 NPs and phoxim treatments using transcriptome sequencing, real-time PCR, and enzyme activity assay. Transcriptome sequencing detected 10 720, 10 641, 10 403, and 10 489 genes for control group, TiO2 NPs group, phoxim group, and TiO2 NPs+phoxim group, respectively. The TiO2 NPs+phoxim group had 705 genes with significantly differential expression (FDR<0.001), among which the antioxidant genes thioredoxin reductase 1 and glutathione S-transferase omega 3 were significantly upregulated. In phoxim group, the expression levels of superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase delta (GSTd), an...Continue Reading

References

Feb 24, 2018·Journal of Economic Entomology·Jinxin LiBing Li
Jul 22, 2018·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Yan PanJin Yu Chieng
May 10, 2019·The Journal of Toxicological Sciences·Do-Hee KimKyung-Soo Chun
Jul 7, 2018·Archives of Insect Biochemistry and Physiology·Hui-Ju GaoYan-Wen Wang

Citations

Mar 20, 1999·The Journal of Biological Chemistry·H R StennickeG S Salvesen
Feb 12, 2002·Pigment Cell Research·Courtney B CaspWayne T McCormack
Jun 27, 2003·Insect Biochemistry and Molecular Biology·G Le GoffR H ffrench-Constant
Dec 21, 2004·Insect Biochemistry and Molecular Biology·Kwang Sik LeeByung Rae Jin
Dec 13, 2005·Obesity Research·Zhihua JiangJohn P McNamara
Oct 7, 2006·Journal of Proteome Research·Xing-hua LiYun-gen Miao
Oct 20, 2007·Nature Reviews. Immunology·Dominique FerrandonJules A Hoffmann
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Nov 19, 2008·Nature Reviews. Genetics·Zhong WangMichael Snyder
Apr 2, 2009·Methods : a Companion to Methods in Enzymology·Brian T Wilhelm, Josette-Renée Landry
Mar 17, 2011·Journal of Economic Entomology·Quan-You YuCheng Lu
Jun 20, 2012·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Sun-Young ParkJinhee Choi
Apr 2, 2013·Archives of Toxicology·Krishnendu SinhaParames C Sil
May 3, 2014·Biological Trace Element Research·Hua ZhangBing Li
Jan 2, 2015·Archives of Environmental Contamination and Toxicology·Ling WangFashui Hong
Jan 27, 2015·Pesticide Biochemistry and Physiology·Z Y GuBing Li

Related Concepts

Metabolic Process, Cellular
Real-Time Polymerase Chain Reaction
Study
Thioredoxin Peroxidase Activity
Fat Pad
Hydrogen Peroxide
Sebacil
Genes
Omega-3 Fatty Acids
Transcription, Genetic

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