Mechanisms of Visceral Organ Crosstalk: Importance of Alterations in Permeability in Rodent Models

The Journal of Urology
B Greenwood-Van MeerveldRobert Hurst

Abstract

The pathophysiology of painful bladder syndrome is poorly understood. However, there is evidence of female predominance and comorbidity with irritable bowel syndrome. Our hypothesis is that cross-sensitization between bladder and colon is due to altered permeability in 1 organ, which affects the other organ. Experiments were performed in anesthetized, ovariectomized female rats. In separate groups protamine sulfate was infused in the bladder or trinitrobenzene sulfonic acid was infused in the colon. Untreated rats served as controls. Bladder and colonic tissue were harvested from all rats 1, 3 and 5 days after treatment. Permeability was assessed in vitro in Ussing chambers by measuring transepithelial electrical resistance and macromolecular flux of fluorescein isothiocyanate-dextran. Exposing the bladder to protamine sulfate induced a significant decrease in bladder transepithelial electrical resistance and an increase in the translocation of fluorescein isothiocyanate across the tissue compared to controls at 1 and 3 days (p <0.05). Colonic tissue from rats with enhanced bladder permeability showed a significant decrease in transepithelial electrical resistance and increase in fluorescein isothiocyanate compared to untreated...Continue Reading

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Citations

Aug 26, 2015·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·R A TownerR E Hurst
May 6, 2016·Journal of Molecular Neuroscience : MN·Beatrice M GirardMargaret A Vizzard
Oct 25, 2016·Experimental Neurology·Beatrice GirardMargaret A Vizzard
Dec 7, 2016·Nature Reviews. Urology·Carsten LeueGommert van Koeveringe
Jun 28, 2016·Journal of Pediatric Gastroenterology and Nutrition·Johana Eyzaguirre-VelásquezMarcela Julio-Pieper
May 23, 2018·The Journal of Pharmacology and Experimental Therapeutics·Ehsan N MohammadiBeverley Greenwood-Van Meerveld
Feb 26, 2016·American Journal of Physiology. Renal Physiology·Robert E HurstBeverley Greenwood-Van Meerveld
Nov 18, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Luke Grundy, Stuart M Brierley
Jan 9, 2019·Frontiers in Neuroscience·Luke GrundyStuart M Brierley
Feb 5, 2021·Pharmacology Research & Perspectives·Rheal A TownerRobert E Hurst
Dec 3, 2020·Frontiers in Psychiatry·Alison Accarie, Tim Vanuytsel

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