Mechanisms underlying the chronotropic effect of angiotensin II on cultured neurons from rat hypothalamus and brain stem

Journal of Neurophysiology
D WangP Posner

Abstract

The chronotropic effect of angiotensin II (Ang II) was studied in cultured neurons from rat hypothalamus and brain stem with the use of the patch-clamp technique. Ang II (100 nM) increased the neuronal spontaneous firing rate from 0.8 +/- 0.3 (SE) Hz in control to 1.3 +/- 0.4 Hz (n = 7, P < 0.05). The amplitude of threshold stimulation was decreased by Ang II (100 nM) from 82 +/- 4 pA to 62 +/- 5 pA (n = 4, P < 0.05). These actions of Ang II were reversed by the angiotensin type 1 (AT1) receptor antagonist losartan (1 microM). In the presence of tetrodotoxin, Ang II (100 nM) significantly increased the frequency and the amplitude of the Cd2+-sensitive subthreshold activity of the cultured neurons. Ang II also stimulated the subthreshold early afterdepolarizations (EADs) to become fully developed action potentials. Similar to the action of Ang II, the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA, 100 nM) increased the firing rate from 0.76 +/- 0.3 Hz to 2.3 +/- 0.5 Hz (n = 6, P < 0.05) and increased the neuronal subthreshold activity. After neurons were intracellularly dialyzed with PKC inhibitory peptide (PKCIP, 5 microM), PMA alone, Ang II alone, or PMA plus Ang II no longer increased the action potent...Continue Reading

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Citations

Sep 2, 2006·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Takao Kubo
Mar 16, 2007·American Journal of Physiology. Cell Physiology·Tomokazu MatsuuraColin Sumners
May 16, 2002·Clinical and Experimental Pharmacology & Physiology·Colin SumnersMingyan Zhu
May 12, 2007·Journal of Neurophysiology·Eduardo AcostaHumberto Cruzblanca
Mar 17, 2009·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Anita IsraelMaider Varela
Dec 2, 2015·Journal of Computational Neuroscience·Warren D AndersonRajanikanth Vadigepalli
May 10, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Melissa A Fleegal, Colin Sumners
Mar 8, 2002·Hypertension·Chengwen SunMohan K Raizada
Feb 9, 2005·Hypertension·Matthew C ZimmermanRobin L Davisson
May 16, 2001·Journal of Neurophysiology·M ZhuP Posner
Jul 12, 2001·Experimental Biology and Medicine·A V FergusonK J Latchford
Jun 13, 2001·American Journal of Physiology. Cell Physiology·S J PanC H Gelband
May 13, 1999·Molecular Neurobiology·E M RichardsC Sumners
May 22, 2007·Stress : the International Journal on the Biology of Stress·Juan M Saavedra, Julius Benicky
Aug 6, 1998·Cellular Signalling·C Sumners, C H Gelband

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