Mechanistic analysis of pause site-dependent and -independent recombinogenic strand transfer from structurally diverse regions of the HIV genome.

The Journal of Biological Chemistry
Suchitra S Derebail, J J DeStefano

Abstract

Retroviral recombinants are generated by strand transfers occurring within internal regions of the viral genome and are a major source of genetic variability. Strand transfer has been linked to "pausing" occurring at secondary structures during synthesis by reverse transcriptase. Yet, weakly structured templates lacking strong pause sites also undergo efficient transfer. In this report, transfer crossover sites on high and low structured templates from the gag-pol frameshift region (GagPol) and the env (Env) regions, respectively, were determined by using a reconstituted in vitro strand transfer assay. The assay tested transfers occurring between a donor and acceptor template over a 150-nucleotide homologous region. The majority of crossovers were in a small 23-nucleotide region near a major pause site on GagPol, clearly indicating a pause-driven mechanism. In contrast, on Env, transfers were more dispersed clustering toward the end of the homologous region. Slowing down polymerization on Env by decreasing the dNTP concentration resulted in crossovers shifting toward the beginning of the homologous region. Removal of a small 38-nucleotide region at the 3'-end of the Env acceptor had a large effect on the level of strand transfe...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H De RocquignyJ L Darlix
Nov 30, 1990·Science·W S Hu, H M Temin
Jan 1, 1990·Annual Review of Genetics·R A Katz, A M Skalka
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W S Hu, H M Temin
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A SrinivasanR A Robinson
Mar 1, 1989·Journal of Virology·F ClavelR Repaske
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·H M Temin
Jul 4, 1997·The Journal of Biological Chemistry·J K KimR A Bambara
Oct 17, 1998·The Journal of Biological Chemistry·H YuJ P Dougherty
Mar 30, 1999·Biochimica Et Biophysica Acta·B I KlasensB Berkhout
Jan 11, 2000·Journal of Virology·A E JetztJ P Dougherty
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·M Negroni, H Buc
May 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·M C WilliamsV A Bloomfield
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·C K HwangV K Pathak
Nov 9, 2001·Annual Review of Genetics·M Negroni, H Buc
May 23, 2002·The Journal of Biological Chemistry·Michele WisniewskiRobert A Bambara
Oct 9, 2002·The Journal of Biological Chemistry·Ricardo H RodaRobert A Bambara
Oct 22, 2002·Journal of Virology·Jianling ZhuangJoseph P Dougherty
Feb 22, 2003·The Journal of Biological Chemistry·Suchitra S DerebailJeffrey J DeStefano
Mar 11, 2004·Proceedings of the National Academy of Sciences of the United States of America·David N LevyGeorge M Shaw
Mar 23, 2004·Journal of Molecular Biology·Michele R S HargittaiKarin Musier-Forsyth
Apr 13, 2004·AIDS·Guowei FangHarold Burger
Jun 1, 2004·The Journal of Biological Chemistry·Christian Lanciault, James J Champoux

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Citations

May 4, 2006·Nucleic Acids Research·Pavlina KonstantinovaBen Berkhout
Sep 28, 2006·Nucleic Acids Research·Heather A BairdMatteo Negroni
Feb 14, 2006·Journal of Virology·Christian Lanciault, James J Champoux
Sep 2, 2009·Microbiology and Molecular Biology Reviews : MMBR·Adewunmi Onafuwa-Nuga, Alice Telesnitsky
Feb 19, 2008·Virus Research·Vandana Purohit BasuRobert A Bambara
Mar 1, 2008·Virus Research·Bertha Cecilia RamirezMatteo Negroni
Oct 12, 2005·Journal of Molecular Biology·Mark Nils HansonRobert A Bambara
Aug 5, 2008·Virus Research·Sonia ZúñigaLuis Enjuanes
Nov 16, 2004·The Journal of Biological Chemistry·Christian Lanciault, James J Champoux
Aug 24, 2006·The Journal of Biological Chemistry·Darwin J OperarioBaek Kim
Mar 6, 2007·The Journal of Biological Chemistry·Vandana PurohitRobert A Bambara
Jan 6, 2007·The Journal of Biological Chemistry·Lu GaoRobert A Bambara
Dec 17, 2010·RNA Biology·Judith G LevinKarin Musier-Forsyth
Nov 18, 2005·The Journal of Biological Chemistry·Román GalettoMatteo Negroni

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