Mechanistic and structural studies of KDM-catalysed demethylation of histone 1 isotype 4 at lysine 26

FEBS Letters
Louise J WalportChristopher J Schofield

Abstract

N-Methylation of lysyl residues is widely observed on histone proteins. Using isolated enzymes, we report mechanistic and structural studies on histone lysine demethylase (KDM)-catalysed demethylation of Nε -methylated lysine 26 on histone 1 isotype 4 (H1.4). The results reveal that methylated H1.4K26 is a substrate for all members of the KDM4 subfamily and that KDM4A-catalysed demethylation of H1.4K26me3 peptide is similarly efficient to that of H3K9me3. Crystallographic studies of an H1.4K26me3:KDM4A complex reveal a conserved binding geometry to that of H3K9me3. In the light of the high activity of the KDM4s on this mark, our results suggest JmjC KDM-catalysed demethylation of H1.4K26 may be as prevalent as demethylation on the H3 tail and warrants further investigation in cells.

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Citations

Jan 26, 2021·Biochemical Society Transactions·Nicolas L Young, Ruhee Dere

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Methods Mentioned

BETA
size exclusion chromatography
NMR
X‐ray
acetylation

Software Mentioned

PHASER
PROCHECK
CNS
HKL2000
PHENIX
COOT
GraphPad

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