Mechanistic Insight into Size-Dependent Enhanced Cytotoxicity of Industrial Antibacterial Titanium Oxide Nanoparticles on Colon Cells Because of Reactive Oxygen Species Quenching and Neutral Lipid Alteration

ACS Omega
Suresh K VermaMrutyunjay Suar

Abstract

This study evaluates the impact of industrially prepared TiO2 nanoparticles on the biological system by using an in vitro model of colon cancer cell lines (HCT116). Industrial synthesis of titanium oxide nanoparticles was mimicked on the lab scale by the high-energy ball milling method by milling bulk titanium oxide particles for 5, 10, and 15 h in an ambient environment. The physiochemical characterization by field emission scanning electron microscopy, dynamic light scattering, and UV-visible spectroscopy revealed alteration in the size and surface charge with respect to increase in the milling time. The size was found to be reduced to 82 ± 14, 66 ± 12, and 42 ± 10 nm in 5, 10, and 15 h milled nano TiO2 from 105 ± 12 nm of bulk TiO2, whereas the zeta potential increased along with the milling time in all biological media. Cytotoxicity and genotoxicity assays performed with HCT116 cell lines by MTT assay, oxidative stress, intracellular lipid analysis, apoptosis, and cell cycle estimation depicted cytotoxicity as a consequence of reactive oxygen species quenching and lipid accumulation, inducing significant apoptosis and genotoxic cytotoxicity. In silico analysis depicted the role of Sod1, Sod2, p53, and VLDR proteins-TiO2 hyd...Continue Reading

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Citations

Nov 18, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Prajita PaulMrutyunjay Suar
Aug 22, 2020·International Journal of Nanomedicine·Kyungeun Kim, Dongwoo Khang
Dec 8, 2020·Nanotoxicology·Abdallah S AbdelsattarMohamed A Helal
Jan 7, 2021·International Journal of Nanomedicine·Reham Samir HamidaMayasar Ibrahim Al-Zaban
Nov 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Amjad Ali KhanArshad Husain Rahmani
Jul 24, 2021·Biological Trace Element Research·Ewa Baranowska-WójcikKlaudia Gustaw
Jul 31, 2018·Chemical Research in Toxicology·Hardik MakkarMrutyunjay Suar

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Methods Mentioned

BETA
dynamic light scattering
scraping
Assay
ELISA
flow cytometry
FCS
Fluorescence microscopy
scanning electron microscopy
transmission electron microscopy
electron microscopy

Software Mentioned

Graphpad Prism
Discovery Studio Visualizer
AutoDock
Cytoscape
FCS Express
LigPlot +
LigPlot
Chimera
FACS express
Gaussian

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