Mechanistic Insight Into the Interaction Between Helicobacter pylori Urease Subunit α and Its Molecular Chaperone Hsp60

Frontiers in Microbiology
Huilin ZhaoXiaofei Ji

Abstract

Helicobacter pylori is the etiologic agent in a variety of gastroduodenal diseases. As its key pathogenic factors, both urease and Hsp60 play important roles in the pathogenesis of H. pylori. Previous studies have suggested that there is close relationship between urease and Hsp60, which implied that Hsp60 may act as a chaperone in urease stabilization and assembly. However, how these two proteins interact remains unclear. In this study, the impact of Hsp60 on urease activity of H. pylori lysate was first detected to confirm the interaction between urease and Hsp60. Pull-down assays further indicated that Hsp60 could bind to UreA subunit but not UreB. Then, the 3D structure of Hsp60 was modeled using I-TASSER to simulate the binding complex with UreA by molecular docking. The results showed that UreA is a perfect fit for the cavity of Hsp60. Analysis of the resulting model demonstrated that at least seven residues of UreA, located on two interfaces, participate in the interaction. Site-directed mutagenesis of these potential residues showed reduced affinity with Hsp60 than the wild type UreA through surface plasmon resonance (SPR) experiments, and D68 appears to have an important role in the affinity. Further analysis also show...Continue Reading

References

May 11, 1992·Infection and Immunity·D J EvansD Y Graham
Nov 1, 1992·Journal of Bacteriology·J W AustinT J Trust
Sep 1, 1991·Infection and Immunity·B E DunnJ G Fox
May 25, 2001·Nature Structural Biology·N C HaB H Oh
Sep 15, 2001·The New England Journal of Medicine·N UemuraR J Schlemper
Dec 12, 2003·World Journal of Gastroenterology : WJG·Yang BaiYa-Li Zhang
Jan 6, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Robert J MaierSusmitha Seshadri
Aug 12, 2009·Biochemical and Biophysical Research Communications·Ching-Yi LinKuang-Wen Liao
Nov 19, 2009·Journal of Biosciences·Shahid KhanShaheryar Iqbal
Oct 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Myoungsun SonBetty Diamond
Aug 30, 2014·Helicobacter·Leonardo H EusebiFranco Bazzoli
Dec 31, 2014·Nature Methods·Jianyi YangYang Zhang
Nov 13, 2015·Postȩpy higieny i medycyny doświadczalnej·Joanna Bajzert, Tadeusz Stefaniak
Jan 13, 2017·Nature Protocols·Dima KozakovSandor Vajda
Sep 29, 2017·Biochemistry and Biophysics Reports·Jose A MendozaMatthew J Swan
Dec 7, 2017·Scientific Reports·Tomoya OkamotoHideaki Itoh

❮ Previous
Next ❯

Citations

Jun 13, 2019·Journal of Cellular Physiology·Masoud KeikhaMohsen Karbalaei
Sep 6, 2019·Helicobacter·Magdalena Chmiela, Juozas Kupcinskas

❮ Previous
Next ❯

Methods Mentioned

BETA
x-ray crystallography
protein folding
pull-down
PCR
gel filtration
protein assay
circular dichroism
chip
pull down

Software Mentioned

PyMOL
MOE
Biacore T100
LOMETS
ClusPro
PDBePISA
Biocore
TASSER
Image J

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.