Mechanistic insights into the dual activities of the single active site of l-lysine oxidase/monooxygenase from Pseudomonas sp. AIU 813.

The Journal of Biological Chemistry
Duangthip TrisriviratPimchai Chaiyen

Abstract

l-Lysine oxidase/monooxygenase (l-LOX/MOG) from Pseudomonas sp. AIU 813 catalyzes the mixed bioconversion of l-amino acids, particularly l-lysine, yielding an amide and carbon dioxide by an oxidative decarboxylation (i.e. apparent monooxygenation), as well as oxidative deamination (hydrolysis of oxidized product), resulting in α-keto acid, hydrogen peroxide (H2O2), and ammonia. Here, using high-resolution MS and monitoring transient reaction kinetics with stopped-flow spectrophotometry, we identified the products from the reactions of l-lysine and l-ornithine, indicating that besides decarboxylating imino acids (i.e. 5-aminopentanamide from l-lysine), l-LOX/MOG also decarboxylates keto acids (5-aminopentanoic acid from l-lysine and 4-aminobutanoic acid from l-ornithine). The reaction of reduced enzyme and oxygen generated an imino acid and H2O2, with no detectable C4a-hydroperoxyflavin. Single-turnover reactions in which l-LOX/MOG was first reduced by l-lysine to form imino acid before mixing with various compounds revealed that under anaerobic conditions, only hydrolysis products are present. Similar results were obtained upon H2O2 addition after enzyme denaturation. H2O2 addition to active l-LOX/MOG resulted in formation of m...Continue Reading

References

Oct 18, 1990·Nature·M Karplus, G A Petsko
Jun 15, 1989·European Journal of Biochemistry·A BöhmerH G Müller
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S YamamotoO Hayaishi
Jan 20, 1972·Biochimica Et Biophysica Acta·M Saito
Apr 20, 2000·Enzyme and Microbial Technology·H OlschewskiG Chemnitius
Dec 26, 2001·Nature Structural Biology·Claudia BindaAndrea Mattevi
Apr 26, 2003·Current Opinion in Chemical Biology·Shelley D Copley
Jun 25, 2004·Nucleic Acids Research·Todd J DolinskyNathan A Baker
Oct 14, 2005·Journal of Computational Chemistry·James C PhillipsKlaus Schulten
May 23, 2006·Journal of Biotechnology·W J H van BerkelM W Fraaije
Aug 31, 2006·Current Opinion in Chemical Biology·Olga KhersonskyDan S Tawfik
Feb 6, 2007·Current Opinion in Chemical Biology·Vivi Joosten, Willem J H van Berkel
Mar 14, 2007·Neurology·C F LippaUNKNOWN DLB/PDD Working Group
Sep 1, 2007·Journal of Biochemistry·Chutintorn SuadeePimchai Chaiyen
Apr 18, 2008·The Journal of Biological Chemistry·Koh IdaHaruo Suzuki
Jul 26, 2008·Biochemistry·Jeerus SucharitakulPimchai Chaiyen
Aug 5, 2009·Archives of Biochemistry and Biophysics·Paul F Fitzpatrick
Jan 12, 2010·Antioxidants & Redox Signaling·Lars M BlankBruno Bühler
Mar 15, 2011·Trends in Biotechnology·Loredano Pollegioni, Gianluca Molla
Jun 19, 2012·Journal of Bioscience and Bioengineering·Kimiyasu IsobeYasuhisa Asano
Jul 24, 2012·Trends in Biochemical Sciences·Pimchai ChaiyenAndrea Mattevi
Sep 13, 2012·Frontiers in Microbiology·Jake AdkinsDavid R Nielsen
May 4, 2013·Applied Microbiology and Biotechnology·Willem P DijkmanMarco W Fraaije
Oct 15, 2013·Basic & Clinical Pharmacology & Toxicology·Mui Li LeeNget Hong Tan
Dec 24, 2013·Archives of Biochemistry and Biophysics·Mieke M E HuijbersWillem J H van Berkel
Apr 30, 1998·The Journal of Physical Chemistry. B·A D MacKerellM Karplus
Jan 13, 2015·Trends in Biochemical Sciences·Shelley D Copley
Jul 29, 2016·The Journal of Biological Chemistry·Janis J FüllerJürgen Moser
Feb 6, 2017·The Journal of Biological Chemistry·Panu PimviriyakulPimchai Chaiyen

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Nov 20, 2020·Metabolomics : Official Journal of the Metabolomic Society·Dongming LvXianbiao Xie
Feb 16, 2021·Biotechnology Advances·Caroline E PaulWillem J H van Berkel
Aug 7, 2021·The Journal of Biological Chemistry·Sayaka SugiuraSohei Ito

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