Mechanistic insights into the host-microbe interaction and pathogen exclusion mediated by the Mucus-binding protein of Lactobacillus plantarum

Scientific Reports
Kumar Siddharth SinghJai Kumar Kaushik

Abstract

Surface adhesins of pathogens and probiotics strains are implicated in mediating the binding of microbes to host. Mucus-binding protein (Mub) is unique to gut inhabiting lactic acid bacteria; however, the precise role of Mub proteins or its structural domains in host-microbial interaction is not well understood. Last two domains (Mubs5s6) of the six mucus-binding domains arranged in tandem at the C-terminus of the Lp_1643 protein of Lactobacillus plantarum was expressed in E. coli. Mubs5s6 showed binding with the rat intestinal mucus, pig gastric mucins and human intestinal tissues. Preincubation of Mubs5s6 with the Caco-2 and HT-29 cell lines inhibited the binding of pathogenic enterotoxigenic E. coli cells to the enterocytes by 68% and 81%, respectively. Pull-down assay suggested Mubs5s6 binding to the host mucosa components like cytokeratins, Hsp90 and Laminin. Mubs5s6 was predicted to possess calcium and glucose binding sites. Binding of Mubs5s6 with these ligands was also experimentally observed. These ligands are known to be associated with pathogenesis suggesting Mub might negotiate pathogens in multiple ways. To study the feasibility of Mubs5s6 delivery in the gut, it was encapsulated in chitosan-sodium tripolyphosphate...Continue Reading

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Citations

May 6, 2020·Critical Reviews in Food Science and Nutrition·Chengcheng RenPaul de Vos
Aug 21, 2020·Microbial Cell Factories·Basavaprabhu H NatarajHariom Yadav
Sep 2, 2020·Journal of Applied Microbiology·H PanwarA K Puniya
Jun 5, 2019·FEMS Microbiology Reviews·Lucie Etienne-MesminStéphanie Blanquet-Diot
Mar 20, 2021·Journal of Applied Microbiology·K S SinghH Panwar
Jul 31, 2021·World Journal of Microbiology & Biotechnology·Aida AitzhanovaAmankeldy Sadanov
Sep 21, 2021·Frontiers in Microbiology·P R ChandhniSunita Grover

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Methods Mentioned

BETA
Gel Filtration
pull-down
Atomic Absorption Spectroscopy
ELISA

Software Mentioned

Modrefiner
COACH
FoldX
Hex
Swiss Model webserver
BioTools
Verify
Yasara
HADDOCK
Tasser

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