Mechanistic investigation in ultrasound induced enhancement of enzymatic hydrolysis of invasive biomass species

Bioresource Technology
Arup Jyoti BorahVijayanand S Moholkar

Abstract

This study has assessed four invasive weeds, viz. Saccharum spontaneum (SS), Mikania micrantha (MM), Lantana camara (LC) and Eichhornia crassipes (EC) for enzymatic hydrolysis prior to bioalcohol fermentation. Enzymatic hydrolysis of pretreated biomasses of weeds has been conducted with mechanical agitation and sonication under constant (non-optimum) conditions. Profiles of total reducible sugar release have been fitted to HCH-1 model of enzymatic hydrolysis using Genetic Algorithm. Trends in parameters of this model reveal physical mechanism of ultrasound-induced enhancement of enzymatic hydrolysis. Sonication accelerates hydrolysis kinetics by ∼10-fold. This effect is contributed by several causes, attributed to intense micro-convection generated during sonication: (1) increase in reaction velocity, (2) increase in enzyme-substrate affinity, (3) reduction in product inhibition, and (4) enhancement of enzyme activity due to conformational changes in its secondary structure. Enhancement effect of sonication is revealed to be independent of conditions of enzymatic hydrolysis - whether optimum or non-optimum.

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Citations

Aug 3, 2016·Bioresource Technology·Alessandro PellisGeorg M Guebitz
Mar 8, 2018·Critical Reviews in Biotechnology·Jiaxin ChenWenyi Dong
Dec 15, 2020·Chemical Society Reviews·Emanuela Calcio GaudinoSilvia Tabasso
Jun 22, 2017·Ultrasonics Sonochemistry·Sneha R Bansode, Virendra K Rathod
Sep 28, 2017·Ultrasonics Sonochemistry·Zumar M A Bundhoo, Romeela Mohee
Aug 12, 2018·Ultrasonics Sonochemistry·Arup Jyoti BorahVijayanand S Moholkar

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