DOI: 10.1101/478818Nov 26, 2018Paper

Mechanistic origin of cell-size control and homeostasis in bacteria

BioRxiv : the Preprint Server for Biology
Fangwei SiSuckjoon Jun

Abstract

Evolutionarily divergent bacteria share a common phenomenological strategy for cell-size homeostasis under steady-state conditions. In the presence of inherent physiological stochasticity, cells following this “adder” principle gradually return to their steady-state size by adding a constant volume between birth and division regardless of their size at birth. However, the mechanism of the adder has been unknown despite intense efforts. In this work, we show that the adder is a direct consequence of two general processes in biology: (1) threshold -- accumulation of initiators and precursors required for cell division to a respective fixed number, and (2) balanced biosynthesis -- maintenance of their production proportional to volume growth. This mechanism is naturally robust to static growth inhibition, but also allows us to “reprogram” cell-size homeostasis in a quantitatively predictive manner in both Gram-negative Escherichia coli and Gram-positive Bacillus subtilis . By generating dynamic oscillations in the concentration of the division protein FtsZ, we were able to oscillate cell size at division and systematically break the adder. In contrast, periodic induction of replication initiator protein DnaA caused oscillations in...Continue Reading

Related Concepts

Birth
Cell Division
Equilibrium
Escherichia coli
Gene Expression
Gram-Negative Bacteria
Peptide Initiation Factors
Research Design
Silicon
Virus Replication

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