PMID: 9177487May 2, 1997Paper

Mechanistic studies of the translational elongation cycle in mammalian mitochondria

Biochimica Et Biophysica Acta
V WoriaxL L Spremulli

Abstract

Polyclonal antibodies have been prepared against both components of the bovine liver mitochondrial translational elongation factor Tu and Ts complex (EF-Tu x Ts(mt)). The antibodies against EF-Tu(mt) cross-react somewhat with Escherichia coli EF-Tu and wheat germ EF-1alpha. The antibodies against EF-Ts(mt) cross-react little, if at all, with E. coli EF-Ts or with EF-Ts from Euglena gracilis chloroplasts. These polyclonal antibodies have been used to investigate the relative amounts of EF-Tu(mt) and EF-Ts(mt) in bovine liver mitochondria and in cultured cells. The results of this analysis suggest that there is a 1:1 ratio of EF-Tu(mt) to EF-Ts(mt) in mammalian mitochondria. Intermediate complexes formed during the elongation cycle of protein synthesis in bovine liver mitochondria have also been investigated. The EF-Tu x Ts(mt) complex is quite resistant to dissociation by guanine nucleotides. This complex will, however, dissociate in the presence of GTP and Phe-tRNA resulting in the formation of a ternary complex comparable to that observed in prokaryotes. Kinetic data suggest that the use of the ternary complex in chain elongation increases the rate of Phe-tRNA binding to ribosomes, suggesting that it is a true intermediate in ...Continue Reading

Citations

May 30, 2001·Advanced Drug Delivery Reviews·I E Scheffler
Nov 16, 2013·Biochemistry. Biokhimii︠a︡·A V KuzmenkoP A Kamenski
Jul 8, 2008·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Ying ZhangGuang-wei Zhong
Apr 17, 2010·Journal of Biomedicine & Biotechnology·Paulien SmitsLambert van den Heuvel
May 26, 2004·Biochimica Et Biophysica Acta·Senyene Eyo Hunter, Linda L Spremulli
Dec 17, 2011·Biochimica Et Biophysica Acta·Brooke E Christian, Linda L Spremulli
Mar 17, 1999·Biochimica Et Biophysica Acta·J W Taanman
Jan 21, 2016·Annual Review of Biochemistry·Martin OttAlan Brown
Mar 15, 2000·Journal of Molecular Biology·G R AndersenJ Nyborg
May 10, 2000·The Journal of Biological Chemistry·Y C CaiL L Spremulli
Feb 18, 2021·Nature Reviews. Molecular Cell Biology·Eva Kummer, Nenad Ban
Sep 11, 2004·Protein Expression and Purification·Kalpana BhargavaLinda L Spremulli

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