Mechanistic studies on the impact of transcription on sequence-specific termination of DNA replication and vice versa.

The Journal of Biological Chemistry
B K MohantyDeepak Bastia

Abstract

Since DNA replication and transcription often temporally and spatially overlap each other, the impact of one process on the other is of considerable interest. We have reported previously that transcription is impeded at the replication termini of Escherichia coli and Bacillus subtilis in a polar mode and that, when transcription is allowed to invade a replication terminus from the permissive direction, arrest of replication fork at the terminus is abrogated. In the present report, we have addressed four significant questions pertaining to the mechanism of transcription impedance by the replication terminator proteins. Is transcription arrested at the replication terminus or does RNA polymerase dissociate from the DNA causing authentic transcription termination? How does transcription cause abrogation of replication fork arrest at the terminus? Are the points of arrest of the replication fork and transcription the same or are these different? Are eukaryotic RNA polymerases also arrested at prokaryotic replication termini? Our results show that replication terminator proteins of E. coli and B. subtilis arrest but do not terminate transcription. Passage of an RNA transcript through the replication terminus causes the dissociation ...Continue Reading

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Citations

Apr 21, 1999·Molecular Microbiology·D E Bussiere, D Bastia
Nov 2, 2002·Molecular Microbiology·L OlavarrietaJ B Schvartzman
Aug 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Deepak BastiaMarc M Greenberg
Jan 26, 2002·Nucleic Acids Research·L OlavarrietaJ B Schvartzman
Jul 12, 2005·Nucleic Acids Research·Paola Loguercio PolosaPalmiro Cantatore
Sep 9, 2005·Microbiology and Molecular Biology Reviews : MMBR·Cameron NeylonNicholas E Dixon
Aug 9, 2001·Proceedings of the National Academy of Sciences of the United States of America·S MuluguD Bastia
Jan 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bidyut K MohantyDeepak Bastia
Jan 24, 2007·Proceedings of the National Academy of Sciences of the United States of America·Marta Fierro-FernándezJorge B Schvartzman
Mar 21, 2009·Molecular Microbiology·Daniel L Kaplan, Deepak Bastia
Jul 7, 2015·Nature Chemical Biology·Bojk A BerghuisNynke H Dekker
May 27, 2015·Nucleic Acids Research·Manjula PandeySmita S Patel
Aug 10, 2010·Cell·Bao Ton-HoangMichael Chandler
Jun 23, 2000·Journal of Molecular Biology·D SantamaríaJ B Schvartzman
Nov 16, 2021·The Journal of Biological Chemistry·Katie H JamesonMichelle Hawkins

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