Mechanistic Study of Bakuchiol-Induced Anti-breast Cancer Stem Cell and in Vivo Anti-metastasis Effects

Frontiers in Pharmacology
Li LiShuk H Cheng

Abstract

Cancer stem cells are involved in cancer establishment, progression, and resistance to current treatments. We demonstrated the in vitro and in vivo anti-breast cancer effect of bakuchiol in a previous study. However, the ability of bakuchiol to target breast cancer stem cells (BCSCs) and inhibit breast cancer metastasis remains unknown. In the current study, we used the cell surface markers CD44 and CD24 to distinguish BCSCs from MCF-7 cells. Bakuchiol inhibited mammosphere formation and aldehyde dehydrogenase activity in BCSCs. Moreover, bakuchiol induced apoptosis and suppressed the mitochondrial membrane potential of BCSCs. Bakuchiol upregulated the expression levels of pro-apoptotic genes, BNIP3 and DAPK2. Bakuchiol induced oxidative stress and altered lipogenesis in BCSCs. In zebrafish xenografts, bakuchiol inhibited breast cancer cell metastasis in vivo. In addition, bakuchiol altered the expression levels of metastasis-related genes through upregulating CK18 and downregulating Notch3, FASN, TGFBR1, and ACVR1B. Our study provides evidence for the anti-breast cancer potential of bakuchiol.

References

Feb 24, 2001·The New England Journal of Medicine·I HedenfalkG Sauter
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Muhammad Al-HajjMichael F Clarke
Apr 23, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ute WoelfleKlaus Pantel
Nov 13, 2004·Breast Cancer Research : BCR·Gabriela DontuMax S Wicha
Apr 19, 2005·Cancer Research·Sharon A VantyghemAnn F Chambers
Jun 3, 2006·Cancer Research·Sucharita BandyopadhyayKounosuke Watabe
Nov 23, 2006·Antioxidants & Redox Signaling·Stefan W RyterAugustine M K Choi
Sep 18, 2007·Stem Cell Reviews·Gillian Farnie, Robert B Clarke
Oct 9, 2007·The Cancer Journal·Madhuri Kakarala, Max S Wicha
Oct 21, 2009·World Journal of Gastroenterology : WJG·Yan GuBo Sun
Mar 30, 2010·Frontiers in Neuroendocrinology·Heather B Patisaul, Wendy Jefferson
Apr 8, 2010·Free Radical Research·Geou-Yarh Liou, Peter Storz
Apr 9, 2010·Future Oncology·Richard FlavinMassimo Loda
Jun 7, 2011·The American Journal of Pathology·Marco A Velasco-VelázquezRichard G Pestell
Feb 7, 2013·Pharmacology & Therapeutics·Yusuke ShiozawaRussell S Taichman
May 7, 2013·The Journal of Endocrinology·Maria Theresa E MontalesRosalia C M Simmen
Sep 21, 2013·Drug Discovery Today. Technologies·Jennifer TatXiao-Yan Wen
Oct 5, 2013·BMC Cancer·Yong TengJohn K Cowell
Nov 19, 2013·Biomolecules & Therapeutics·Yhun Yhong SheenJeong-Seok Nam
Feb 13, 2014·International Journal of Biological Sciences·Junqin LiHua Li
May 30, 2014·The American Journal of Clinical Nutrition·Janet M PaveseRaymond C Bergan
Oct 11, 2014·World Journal of Clinical Oncology·Iqra BilalSaffron Whitehead
Aug 19, 2015·Cancer Research·Silja HeilmannRichard White
Oct 16, 2015·Nature·Elena PiskounovaSean J Morrison
Dec 5, 2015·Cell Metabolism·Maria Peiris-PagèsMichael P Lisanti
Sep 3, 2016·Seminars in Cancer Biology·Gaia CantelliVictoria Sanz-Moreno
Mar 5, 2017·Seminars in Cancer Biology·Claudia PeitzschAnna Dubrovska
Mar 10, 2017·Expert Opinion on Drug Discovery·Hannah K BrownTimothy Ja Chico
May 20, 2017·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Angela B MariottoMusa Mayer

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Methods Mentioned

BETA
flow cytometry
Assay
PCR
xenograft

Software Mentioned

SPSS
FACS Diva
lightsheet
SigmaPlot

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