Abstract
Whole-organ engineering-based on the functional repopulation of acellular whole-organ scaffolds derived from perfusion-based in toto decellularization of the specific organ system-is one of the most promising fields in tissue engineering. However, to date, we still have hardly any insights into the process of perfusion-based scaffold generation itself, with human-scale scaffolds usually obtained by adoption of small animal decellularization models, although those organs are of decreased biomass and potentially different biological characteristics. Therefore, in this study we analyzed perfusion-based human-scale whole-heart decellularization by evaluating and comparing the dynamics of biomass discharge and its kinetic characteristics during in toto decellularization of ovine and rodent hearts, while introducing a theoretical model of biomass depletion during perfusion-based whole-heart decellularization. Our results suggest highly varying process characteristics for the in toto decellularization of individual human-scale organs, such as protein discharge kinetics or time-dependent viscoelasticity of formed debris, despite seemingly consistent inter-sample decellularization efficacy, as evaluated by conventional disruptive analys...Continue Reading
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