Mechanochemical aspects of axonemal dynein activity studied by in vitro microtubule translocation

Biophysical Journal
T HamasakiP Satir

Abstract

We have determined the relationship between microtubule length and translocation velocity from recordings of bovine brain microtubules translocating over a Paramecium 22S dynein substratum in an in vitro assay chamber. For comparison with untreated samples, the 22S dynein has been subjected to detergent and/or to pretreatments that induce phosphorylation of an associated 29 kDa light chain. Control and treated dyneins have been used at the same densities in the translocation assays. In any given condition, translocation velocity (v) shows an initial increase with microtubule length (L) and then reaches a plateau. This situation may be represented by a hyperbola of the general form v = aL/(L+b), which is formally analogous to the Briggs-Haldane relationship, which we have used to interpret our data. The results indicate that the maximum translocation velocity Vo(= a) is increased by pretreatment, whereas the length constant KL(= b), which corresponds to Km, does not change with pretreatment, implying that the mechanochemical properties of the pretreated dyneins differ from those of control dyneins. The conclusion that KL is constant for defined in vitro assays rules out the possibility that the velocity changes seen are caused b...Continue Reading

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Citations

Jul 12, 2002·Cell Motility and the Cytoskeleton·Charles B Lindemann
Jan 19, 2000·Molecular Biology of the Cell·Y WadaP Satir
Jan 17, 2003·Molecular Biology of the Cell·Charles GuerraPeter Satir
Apr 18, 2001·Trends in Cell Biology·W S Sale, K L Barkalow
Nov 2, 1999·The Journal of Physiology·M Salathe, R J Bookman
Apr 13, 2010·Journal of Theoretical Biology·Christian CibertGuy Bonnet
Feb 7, 2015·Biophysical Journal·Joshua D AlperJonathon Howard
Feb 7, 1998·Cell Motility and the Cytoskeleton·H Wang, P Satir
Apr 19, 2008·Cell Motility and the Cytoskeleton·David P LorchAlan J Hunt
Jan 13, 1997·The Journal of Cell Biology·G Habermacher, W S Sale
Jan 5, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P Satir
Mar 29, 2001·The Journal of Biological Chemistry·S T ChristensenT Hamasaki
Dec 18, 2004·Cell Motility and the Cytoskeleton·Raviraja N Seetharam, Peter Satir
Jan 12, 2010·Nanomedicine : Nanotechnology, Biology, and Medicine·Michael J AllenRod Balhorn

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