Mechanosensitive channel of Thermoplasma, the cell wall-less archaea: cloning and molecular characterization

Cell Biochemistry and Biophysics
A Kloda, B Martinac

Abstract

By using a functional approach of reconstituting detergent-solubilized membrane proteins into liposomes and following their function in patch-clamp experiments, we identified a novel mechanosensitive (MS) channel in the thermophilic cell wall-less archaeon Thermoplasma volcanium. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of the enriched protein fractions revealed a band of approx 15 kDa comparable to MscL, the bacterial MS channel of large conductance. 20 N-terminal residues determined by protein microsequencing, matched the sequence to an unknown open reading frame in the genome of a related species Thermoplasma acidophilum. The protein encoded by the T. acidophilum gene was cloned and expressed in Escherichia coli and reconstituted into liposomes. When examined for function, the reconstituted protein exhibited properties typical of an MS ion channel: 1) activation by negative pressure applied to the patch-clamp pipet, 2) blockage by gadolinium, and 3) activation by the anionic amphipath trinitrophenol. In analogy to the nomenclature used for bacterial MS channels, the MS channel of T acidophilum was termed MscTA. Secondary structural analysis indicated that similar to MscL, the T. acidophilum MS pro...Continue Reading

Citations

Nov 8, 2006·European Biophysics Journal : EBJ·Evgeny Petrov, Boris Martinac
Oct 6, 2006·Pflügers Archiv : European journal of physiology·O P Hamill
Sep 13, 2008·Cell Biochemistry and Biophysics·Yuye TangXi Chen
May 7, 2003·Progress in Biophysics and Molecular Biology·Boris Martinac, Anna Kloda
Feb 16, 2005·Astrobiology·Andrew PohorilleMichael A Wilson
Oct 17, 2008·Physiological Reviews·Boris MartinacChing Kung
Apr 5, 2005·Archaea : an International Microbiological Journal·Anna Kloda, Boris Martinac
Mar 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Boris Martinac, Owen P Hamill
Oct 20, 2010·The Journal of Cell Biology·Ellen A LumpkinAislyn M Nelson
Dec 30, 2014·International Journal of Molecular Sciences·Takuya KawamuraHiroyuki Hori
Feb 18, 2010·Protein Science : a Publication of the Protein Society·Zhenfeng LiuDouglas C Rees
Jan 3, 2006·Biophysical Journal·Anna KlodaBoris Martinac
Sep 26, 2003·Biophysical Journal·Justin Gullingsrud, Klaus Schulten
Jul 31, 2007·Biochimica Et Biophysica Acta·Ben Corry, Boris Martinac
Sep 24, 2004·Physiological Reviews·Popi Syntichaki, Nektarios Tavernarakis
Jun 21, 2019·Protein and Peptide Letters·Jian-Ping HuShan Chang
Jul 17, 2020·Frontiers in Cell and Developmental Biology·Roberto GaetaniElisa Messina
May 26, 2004·Journal of Cell Science·Boris Martinac
Nov 28, 2008·Biochemistry·Jérôme LacroixLaurent Counillon
Apr 28, 2001·Biophysical Journal·J GullingsrudK Schulten

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.