Mechanosensitive closed-closed transitions in large membrane proteins: osmoprotection and tension damping.

Biophysical Journal
Pierre-Alexandre BoucherBéla Joós

Abstract

Multiconformation membrane proteins are mechanosensitive (MS) if their conformations displace different bilayer areas. Might MS closed-closed transitions serve as tension buffers, that is, as membrane "spandex"? While bilayer expansion is effectively instantaneous, transitions of bilayer-embedded MS proteins are stochastic (thermally activated) so spandex kinetics would be critical. Here we model generic two-state (contracted/expanded) stochastic spandexes inspired by known bacterial osmovalves (MscL, MscS) then suggest experimental approaches to test for spandex-like behaviors in these proteins. Modeling shows: 1), spandex kinetics depend on the transition state location along an area reaction coordinate; 2), increasing membrane concentration of a spandex right-shifts its midpoint (= tension-Boltzmann); 3), spandexes with midpoints below the activating tension of an osmovalve could optimize osmovalve deployment (required: large midpoint, barrier near the expanded state); 4), spandexes could damp bilayer tension excursions (required: midpoint at target tension, and for speed, barrier halfway between the contracted and expanded states; the larger the spandex Delta-area, the more precise the maintenance of target tension; higher ...Continue Reading

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Citations

Sep 21, 2013·ACS Nano·Romain SalvaSébastien Lecommandoux
Jun 2, 2010·The Journal of General Physiology·Vladislav BelyySergei Sukharev
Jun 26, 2013·The Journal of General Physiology·Ian RoweSergei Sukharev
Mar 20, 2012·PloS One·Maja Bialecka-FornalRob Phillips
Feb 14, 2015·PloS One·Catherine E MorrisBéla Joós
Nov 24, 2018·Scientific Reports·Meghedi BabakhanianWarren Grundfest
Oct 29, 2021·The Journal of Physical Chemistry. B·David GomezGlen M Hocky

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