PMID: 7537813Dec 1, 1994Paper

Mechanosensitive ion channels in skeletal muscle from normal and dystrophic mice

The Journal of Physiology
Alfredo Franco-Obregón, J B Lansman

Abstract

1. We examined the activity of single mechanosensitive ion channels in recordings from cell-attached patches on myoblasts, differentiated myotubes and acutely isolated skeletal muscle fibres from wild-type and mdx and dy mutant mice. The experiments were concerned with the role of these channels in the pathophysiology of muscular dystrophy. 2. The predominant form of channel activity recorded with physiological saline in the patch electrode arose from an approximately 25 pS mechanosensitive ion channel. Channel activity was similar in undifferentiated myoblasts isolated from all three strains of mice. By contrast, channel activity in mdx myotubes was approximately 3-4 times greater than in either wild-type or dy myotubes and arose from a novel mode of mechanosensitive gating. 3. Single mechanosensitive channels in acutely isolated flexor digitorum brevis fibres had properties indistinguishable from those of muscle cells grown in tissue culture. The channel open probability in mdx fibres was approximately 2 times greater than the activity recorded from wild-type fibres. The overall level of activity in fibres, however, was roughly an order of magnitude smaller than in myoblasts or myotubes. 4. Histological examination of the fle...Continue Reading

Citations

Apr 18, 2002·Neuroscience Letters·Clarisse VandebrouckChristian Cognard
Feb 13, 2001·Neuromuscular Disorders : NMD·C VandebrouckG Raymond
Nov 10, 2009·Molecular Neurobiology·Gonneke S K PilgramJasprina N Noordermeer
Jun 1, 2014·The Journal of Physiology·Nhi Tan, Jeffry B Lansman
Nov 6, 2004·The Journal of Physiology·Ella W YeungDavid G Allen
Jan 27, 2007·The Journal of Physiology·Thomas M Suchyna, Frederick Sachs
Nov 5, 2003·The Journal of Physiology·O FriedrichR H A Fink
Jul 11, 2006·The Journal of Physiology·Thomas DucretPhilippe Gailly
Jun 9, 2009·Pharmacology & Therapeutics·Ryuji InoueYasuhiro Kawarabayashi
Jul 2, 1998·Biophysical Journal·J Akinlaja, F Sachs
Dec 13, 2006·Toxicon : Official Journal of the International Society on Toxinology·Charles L BowmanFrederick Sachs
Oct 11, 2005·Physical Medicine and Rehabilitation Clinics of North America·Jay J HanJoseph N Kornegay
Jan 13, 2011·The American Journal of Pathology·Louise H JørgensenHanns Lochmüller
Feb 22, 2012·Current Opinion in Pharmacology·Philippe Gailly
May 26, 2015·Journal of Molecular Biology·James B MorrisseyPenney M Gilbert
Sep 12, 2013·PloS One·Haixia HuangThomas M Suchyna
Jun 1, 1996·British Journal of Pharmacology·W J LeijendekkerU T Rüegg
Feb 26, 2004·The Journal of General Physiology·Bradley S Launikonis, D George Stephenson
Feb 12, 2004·Science's STKE : Signal Transduction Knowledge Environment·Sergei Sukharev, David P Corey
Sep 18, 2002·The Journal of Cell Biology·Clarisse VandebrouckPhilippe Gailly
Oct 6, 2005·Physical Biology·V S Markin, F Sachs
Feb 19, 2000·Microscopy Research and Technique·A A HackE M McNally
Nov 11, 2005·Muscle & Nerve·Jay J HanLawrence R Robinson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.