Mechanosensitive transient receptor potential vanilloid type 1 channels contribute to vascular remodeling of rat fistula veins

Journal of Vascular Surgery
Yih-Sharng ChenMing-Chieh Ma

Abstract

We previously showed that matrix metalloproteinases (MMPs) contribute to tremendous blood flow-induced venous wall thickening during the maturation of an arteriovenous fistula (AVF). However, how veins in the fistula sense a dramatic change in the blood flow remains unknown. Because mechanosensitive transient receptor potential vanilloid channels (TRPVs) are present in the endothelium, we examined whether the Ca2+-permeable TRPVs play a role in remodeling of fistula veins. The fistula veins were generated at femoral AVF of Wistar rats. Changes in the hemodynamics and the width and internal radius of the iliac vein were studied at 3, 7, 14, and 28 days, then the iliac vein was removed and examined for changes in wall thickness and protein or mRNA expression by immunofluorecent stain, Western blot, or real time PCR. Changes in MMP2 activity was examined by gelatin zymography. Two ligatures were performed in iliac vein to prevent venodilatation to confirm the effect of dramatic changes in hemodynamics on TRPV expression. The specific role of TRPV was studied in another group of fistula veins given with capsazepine via a subcutaneous mini-osmotic pump for 28 days. The fistula veins demonstrated high flow/wall shear stress (WSS), wa...Continue Reading

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Citations

Aug 22, 2014·Proceedings of the National Academy of Sciences of the United States of America·Na LuoYang Sun
Apr 3, 2015·Vascular Medicine·Yung-Wei Chi, Joseph D Raffetto
Apr 3, 2015·Physiological Reviews·Scott Earley, Joseph E Brayden
May 19, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Nga Ling KoGeorge Osol
Mar 17, 2021·The Journal of Pharmacology and Experimental Therapeutics·Yixi LiuChe-Ping Cheng

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