Mechanosensitivity is an essential component of phototransduction in vertebrate rods

PLoS Biology
Ulisse BoccheroVincent Torre

Abstract

Photoreceptors are specialized cells devoted to the transduction of the incoming visual signals. Rods are able also to shed from their tip old disks and to synthesize at the base of the outer segment (OS) new disks. By combining electrophysiology, optical tweezers (OTs), and biochemistry, we investigate mechanosensitivity in the rods of Xenopus laevis, and we show that 1) mechanosensitive channels (MSCs), transient receptor potential canonical 1 (TRPC1), and Piezo1 are present in rod inner segments (ISs); 2) mechanical stimulation-of the order of 10 pN-applied briefly to either the OS or IS evokes calcium transients; 3) inhibition of MSCs decreases the duration of photoresponses to bright flashes; 4) bright flashes of light induce a rapid shortening of the OS; and 5) the genes encoding the TRPC family have an ancient association with the genes encoding families of protein involved in phototransduction. These results suggest that MSCs play an integral role in rods' phototransduction.

References

Dec 1, 1973·The Journal of Physiology·R Zuckerman
Jun 5, 2001·Nature Reviews. Neuroscience·D E ClaphamC Strübing
Mar 28, 2002·Physiological Reviews·Baruch Minke, Boaz Cook
Aug 31, 2002·Biophysical Journal·Chunhe ChenYiannis Koutalos
Oct 9, 2002·Neuron·Marie E BurnsDenis A Baylor
Dec 5, 2003·Nature·David E Clapham
Jan 25, 2005·Nature Cell Biology·Rosario MarotoOwen P Hamill
Feb 25, 2005·Science's STKE : Signal Transduction Knowledge Environment·Craig Montell
Aug 1, 2006·The Review of Scientific Instruments·Keir C Neuman, Steven M Block
Oct 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Maria A SpassovaDonald L Gill
May 31, 2007·The Journal of Cell Biology·Stephan NickellKrzysztof Palczewski
May 21, 2008·Journal of Proteome Research·Isabella PanfoliGiovanni Candiano
Oct 29, 2008·The Journal of General Physiology·Benjamin R MyersChing Kung
Mar 3, 2010·Annual Review of Biophysics·Jóhanna Arnadóttir, Martin Chalfie
Mar 6, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Owen P Gross, Marie E Burns
Jun 24, 2011·Biochemistry·Chilman BaePhilip A Gottlieb
Jun 8, 2012·The Journal of Physiology·Tünde MolnarDavid Križaj
Oct 16, 2012·Science·Roger C Hardie, Kristian Franze
Oct 15, 2013·Cell·Patrick Delmas, Bertrand Coste
Jan 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Timothy ConnellyMinghong Ma
Jan 17, 2015·Cell Calcium·Ian R BoothLaura Lehtovirta-Morley
Jul 1, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Justin BerryCarter Cornwall
Jan 12, 2017·Biophysical Journal·Radhakrishnan GnanasambandamThomas M Suchyna
Mar 23, 2017·Proceedings of the National Academy of Sciences of the United States of America·Pengfei ZhangEdward N Pugh
Jun 29, 2018·Frontiers in Cellular Neuroscience·Fabio FalleroniDan Cojoc
Sep 28, 2018·Open Biology·Trevor D Lamb, David M Hunt
Jan 9, 2019·Translational Vision Science & Technology·Yiming LuXincheng Yao
Mar 7, 2019·Investigative Ophthalmology & Visual Science·Ulisse BoccheroOrson L Moritz

❮ Previous
Next ❯

Citations

Aug 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yunzhen LiVincent Torre
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Isaac C D LentonItia A Favre-Bulle

❮ Previous
Next ❯

Methods Mentioned

BETA
optical tweezers
Fluorescence
force measurement
mechanical dissociation

Software Mentioned

MATLAB
HCImage
Micromanager
GraphPad
GraphPad Prism
SigmaPlot
ImageJ
Clampfit

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Related Papers

Annual Review of Neuroscience
E A Schwartz
Cold Spring Harbor Symposia on Quantitative Biology
Vincent TorreM Campani
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Osamu Hisatomi, Fumio Tokunaga
Investigative Ophthalmology & Visual Science
Eduardo SolessioR B Barlow
© 2021 Meta ULC. All rights reserved