Mechanosensitivity of mitochondrial large-conductance calcium-activated potassium channels

Biochimica Et Biophysica Acta. Bioenergetics
Agnieszka WalewskaPiotr Koprowski

Abstract

Potassium channels have been discovered in the inner mitochondrial membrane of various cells. These channels can regulate the mitochondrial membrane potential, the matrix volume, respiration and reactive species generation. Therefore, it is believed that their activation is cytoprotective in various tissues. In our study, the single-channel activity of a large-conductance calcium-activated potassium channel (mitoBKCa) was measured by the patch-clamp technique on mitoplasts derived from mitochondria isolated from human glioma U-87 MG cells. Here, we show for the first time that mechanical stimulation of mitoBKCa channels results in an increased probability of channel opening. However, the mechanosensitivity of mitoBKCa channels was variable with some channels exhibiting no mechanosensitivity. We detected the expression of mechanosensitive BKCa-STREX exon in U-87 MG cells and hypotesize, based on previous studies demonstrating the presence of multiple BKCa splice variants that variable mechanosensitivity of mitoBKCa could be the result of the presence of diverse BKCa isoforms in mitochondria of U-87 MG cells. Our findings indicate the possible involvement of the mitoBKCa channel in mitochondria activities in which changes in memb...Continue Reading

Citations

Nov 16, 2018·British Journal of Pharmacology·Luigi LeanzaIldiko Szabo
Dec 17, 2019·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Lena R BartellMichelle L Delco
Nov 17, 2019·International Journal of Molecular Sciences·Michał LaskowskiBogusz Kulawiak
Aug 23, 2020·Biomolecules·Antoni WrzosekAdam Szewczyk
Feb 16, 2021·Frontiers in Cell and Developmental Biology·Eunhee KimKyeong Sook Choi
Jan 11, 2021·Biochimica Et Biophysica Acta. Bioenergetics·Shane Austin, Karin Nowikovsky
Jun 24, 2020·The International Journal of Biochemistry & Cell Biology·Daria RotkoBogusz Kulawiak
Nov 29, 2020·Bio Systems·Agata Wawrzkiewicz-JałowieckaPiotr Bednarczyk
May 1, 2021·Pharmacology & Therapeutics·Vanessa ChecchettoIldiko Szabo
May 7, 2021·Channels·Ana L González-CotaEnrique Balderas
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aleksandra SekPiotr Bednarczyk
Apr 4, 2020·Ecotoxicology and Environmental Safety·Elena A Belyaeva, Tatyana V Sokolova

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