Meconium exposure dependent cell death and apoptosis in human alveolar epithelial cells

Pediatric Pulmonology
Mei-Jy JengRen-Bin Tang

Abstract

Alveolar epithelial cells of neonates are directly exposed to aspirated meconium during meconium aspiration syndrome (MAS). This study was designed to investigate the influence of quantity and time of meconium exposure on the cell viability and caspase activity in type II human alveolar epithelial cells. Human alveolar epithelial cells were incubated with human meconium suspension at different concentrations and for different times. Cell viability and DNA fragmentation were investigated together with caspases activity and the amount of Bcl-2 protein present. We found that cell viability was significantly lower in cells exposed to a higher concentration of meconium. This was also true for cells exposed to meconium for longer. Significantly higher DNA fragmentation, an approximately two- to fivefold increase, was observed in cells that had been exposed to higher (5% and 10%) concentration of meconium compared to those treated with lower (0.1% and 1%) concentrations (P < 0.05). The activity of most apoptotic initiators (caspase 2, 8, 9, 10) and effectors (caspase 3, 6) were found to be significantly higher in cells subject to greater meconium exposure compared to cells with no or minor meconium exposure. The level of Bcl-2 was als...Continue Reading

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Citations

Jul 11, 2012·Gastroenterology Research and Practice·Sotirios D GeorgopoulosStylianos Karatapanis
Dec 19, 2015·Pediatric Research·Jana Kopincova, Andrea Calkovska
Sep 16, 2016·Histochemistry and Cell Biology·Hanna MüllerJan Mollenhauer
Dec 21, 2018·Expert Review of Respiratory Medicine·Andrea CalkovskaMirko Zibolen
Apr 30, 2020·American Journal of Respiratory Cell and Molecular Biology·Chiara AutilioDaniele De Luca
Dec 14, 2018·Cell Proliferation·Zeng ZhaolinWang Zuo
Mar 8, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jana KopincovaDaniela Mokra
Mar 21, 2019·Journal of the Chinese Medical Association : JCMA·Ying ChenMei-Jy Jeng

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