Media calcium attenuates mitochondrial 1,25(OH)2D production in phosphorus or vitamin D-deprived rats

Pediatric Research
T O Carpenter, B Ellis

Abstract

Although PTH and hypophosphatemia are the best known stimulators of 25-hydroxyvitamin D-1 alpha-hydroxylase, 1,25(OH)2D3 production in rats and humans can be modulated by circulating calcium, independent of PTH. To test whether calcium modulates this function directly in mitochondria, we examined effects of calcium on 1 alpha-hydroxylase in isolated mitochondrial preparations under basal and stimulated conditions. Rats were fed a low phosphorus (or matched control) diet for 4 or 7 d or a vitamin D-deficient (or matched control) diet for 2, 4, or 7 wk. Renal mitochondria were isolated and assayed for 1 alpha-hydroxylase activity in the presence or absence of added calcium. Calcium did not alter 1 alpha-hydroxylase in rats on control diets. After 4 d of low phosphorus diet, 1 alpha-hydroxylase was increased 2-fold over basal activity; media calcium prevented this stimulatory response. By 7 d the calcium effect was not evident. After 4 wk of vitamin D deprivation, activity was approximately 30-fold greater than controls; calcium reduced this response significantly (15-fold). A significant, but less marked inhibition of activity by calcium was present in rats subjected to 7 wk of vitamin D deprivation. Extramitochondrial calcium ca...Continue Reading

Citations

Jan 23, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Alexandra MuscherKorinna Huber
Feb 24, 2001·Bioscience, Biotechnology, and Biochemistry·R MasuyamaK Suzuki
Oct 1, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Bridget E YoungKimberly O O'Brien
Apr 1, 1997·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·G VezzoliG Bianchi

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