Mediation of endothelial cell damage by serine proteases, but not superoxide, released from antineutrophil cytoplasmic antibody-stimulated neutrophils

Arthritis and Rheumatism
X LuG B Nash

Abstract

To evaluate potential mediators of endothelial cell injury in systemic vasculitis associated with antineutrophil cytoplasmic antibodies (ANCAs), we investigated the factors controlling the neutrophil respiratory burst and endothelial release of von Willebrand factor (vWF) during neutrophil-endothelial cell interactions. Superoxide release from neutrophils binding to purified P-selectin or to tumor necrosis factor-activated endothelial cells was measured under flow or static conditions using the superoxide dismutase (SOD)-inhibitable reduction of ferricytochrome c. Neutrophils were activated with fMLP, normal IgG, or ANCA IgG. Enzyme-linked immunosorbent assay was used to measure vWF. Serine protease activity was measured enzymatically. ANCA IgG or fMLP induced superoxide release when perfused over neutrophils that were rolling over P-selectin, but not those that were binding to endothelial cells. In static assays, endothelial cells inhibited superoxide production by neutrophils. Adenosine inhibited the respiratory burst, and, in cocultures, adenosine deaminase overcame the inhibitory effects of endothelial cells. Serine proteases were released during activated neutrophil-endothelial cell coculture. There was enhanced release of...Continue Reading

References

Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R J FalkJ C Jennette
Sep 1, 1986·The Journal of Clinical Investigation·B N CronsteinR Hirschhorn
Dec 1, 1984·Arthritis and Rheumatism·S R NusinowJ G Curd
Jan 16, 2002·Current Opinion in Rheumatology·Caroline O S SavageMark Holland
Dec 9, 2003·The Journal of Clinical Investigation·Tieming ZhaoGary M Bokoch
Dec 17, 2003·Kidney International·William F PendergraftGloria A Preston
Jan 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jussi NiemeläSirpa Jalkanen

❮ Previous
Next ❯

Citations

Oct 28, 2008·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Silvia M UriarteRichard A Ward
Jan 24, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Philipp KümpersMarion Haubitz
Jul 16, 2009·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Mark A LittleLorraine Harper
Mar 23, 2011·Current Opinion in Nephrology and Hypertension·J Charles JennetteAdil H Gasim
Apr 23, 2013·Rheumatology International·Ali MotaSaeed Najavand
Jul 9, 2014·Nature Reviews. Rheumatology·J Charles Jennette, Ronald J Falk
Apr 30, 2014·Seminars in Immunopathology·J Charles Jennette, Ronald J Falk
Oct 17, 2007·Seminars in Immunopathology·Stephen R Holdsworth, Peter G Tipping
Aug 21, 2008·Scandinavian Journal of Clinical and Laboratory Investigation. Supplementum·Ralph Kettritz
Jun 11, 2015·Kidney International·Uwe JerkeRalph Kettritz
Dec 15, 2010·Kidney International·Benjamin WildeJan Willem Cohen Tervaert
Aug 7, 2010·Rheumatic Diseases Clinics of North America·Julia FlintCaroline O S Savage
Mar 17, 2007·La Presse médicale·Christian Pagnoux, Luis Teixeira
Jul 30, 2009·APMIS. Supplementum·Caroline O S Savage
Aug 7, 2012·Clinical and Experimental Immunology·R Kettritz
May 29, 2007·Arthritis and Rheumatism·Peter LamprechtDieter Kabelitz
Oct 31, 2007·Arthritis and Rheumatism·Lars C HuberSteffen Gay
Jul 12, 2014·Journal of the American Society of Nephrology : JASN·Adrian SchreiberRalph Kettritz
Jul 9, 2017·Journal of the American Society of Nephrology : JASN·Mira ChoiRalph Kettritz
Apr 21, 2006·Journal of the American Society of Nephrology : JASN·Matthew David MorganCaroline Savage
Jul 20, 2007·Journal of the American Society of Nephrology : JASN·Soumeya BrachemiLise Halbwachs-Mecarelli
Jun 18, 2017·Journal of the American Society of Nephrology : JASN·Dawn J CasterKenneth R McLeish
May 22, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Rafal FudalaAnna K Kurdowska
Jun 3, 2017·Arthritis & Rheumatology·Ryosuke HiwaHisashi Arase
Jan 29, 2020·Journal of Leukocyte Biology·Sergio D Catz, Kenneth R McLeish
Jul 1, 2007·Expert Review of Clinical Immunology·Alastair J FerraroCaroline O Savage
Dec 28, 2019·Journal of the American Society of Nephrology : JASN·Marilina AntonelouUNKNOWN RAVE-ITN Investigators

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

© 2022 Meta ULC. All rights reserved