Abstract
Mediator complex subunit 16 (MED16) is a component of the mediator complex and functions as a coactivator in transcriptional events at almost all RNA polymerase II-dependent genes. In this study, we report that the expression of MED16 is markedly decreased in papillary thyroid cancer (PTC) tumors compared with normal thyroid tissues. In vitro, MED16 overexpression in PTC cells significantly inhibited cell migration, enhanced sodium/iodide symporter expression and iodine uptake, and decreased resistance to radioactive 131I (RAI). Conversely, PTC cells in which MED16 had been further knocked down (MED16KD) exhibited enhanced cell migration, epithelial-mesenchymal transition, and RAI resistance, accompanied by decreased sodium/iodide symporter levels. Moreover, cell signaling through transforming growth factor β (TGF-β) was highly activated after the MED16 knockdown. Similar results were obtained in MED12KD PTC cells, and a co-immunoprecipitation experiment verified interactions between MED16 and MED12 and between MED16 and TGF-βR2. Of note, the application of LY2157299, a potent inhibitor of TGF-β signaling, significantly attenuated MED16KD-induced RAI resistance both in vitro and in vivo In conclusion, our findings indicate that...Continue Reading
References
Mar 1, 1989·The Journal of Clinical Investigation·B Grubeck-LoebensteinH Kassal
Aug 28, 1999·Endocrinology·A FranzénN E Heldin
Feb 18, 2003·Endocrine Reviews·Orsolya DohánNancy Carrasco
Aug 2, 2003·Journal of the American College of Surgeons·Margo ShoupAshok R Shaha
Oct 31, 2003·Oncogene·Susana B BravoClara V Alvarez
Nov 19, 2003·The Journal of Biological Chemistry·Eugenia CostamagnaPilar Santisteban
Sep 22, 2005·The Journal of Clinical Endocrinology and Metabolism·Mingzhao XingPaul W Ladenson
May 11, 2006·The Journal of Clinical Endocrinology and Metabolism·C DuranteM Schlumberger
Jun 3, 2009·Cancer Research·Julio C Ricarte-FilhoJames A Fagin
Jul 9, 2009·Cancer Research·Thiruvengadam ArumugamWoonyoung Choi
Oct 29, 2009·Thyroid : Official Journal of the American Thyroid Association·UNKNOWN American Thyroid Association (ATA) Guidelines Taskforce on Thyroid Nodules and Differentiated Thyroid CancerR Michael Tuttle
Oct 29, 2009·Cancer Research·Garcilaso Riesco-EizaguirrePilar Santisteban
Dec 17, 2009·Cancer Metastasis Reviews·David J McConkeyColin Dinney
Mar 2, 2010·Oncogene·H OchiaiT Kamijo
Aug 7, 2010·Hormones : International Journal of Endocrinology and Metabolism·Leonard Wartofsky
Jul 8, 2011·Cell Death & Disease·M SaxenaA Rangarajan
Mar 9, 2012·Journal of Cell Science·Samy LamouilleRik Derynck
May 17, 2012·Cancer Discovery·Ethan CeramiNikolaus Schultz
Jul 26, 2012·Thyroid : Official Journal of the American Thyroid Association·Iain J NixonIan Ganly
Nov 28, 2012·Cell·Sidong HuangRené Bernards
Apr 4, 2013·Science Signaling·Jianjiong GaoNikolaus Schultz
May 30, 2013·Nature Genetics·UNKNOWN GTEx Consortium
Aug 27, 2013·Cell Cycle·Diede BrunenRené Bernards
Dec 18, 2013·Biochimica Et Biophysica Acta·Concetta SchianoClaudio Napoli
Nov 25, 2014·Cell·UNKNOWN Cancer Genome Atlas Research Network
Jan 15, 2015·Molecular Medicine Reports·Xinyong ZhuWen Tian
Feb 6, 2015·CA: a Cancer Journal for Clinicians·Lindsey A TorreAhmedin Jemal
Jul 3, 2015·International Journal of Molecular Medicine·Lei ZhaoXiujuan Qu
Aug 13, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Siyuan MaGuijun Qin
Apr 7, 2016·Oncotarget·Isabella SyringSven Perner
Feb 8, 2018·Annual Review of Pathology·Vivek Mittal
Oct 24, 2018·International Journal of Cancer. Journal International Du Cancer·J FerlayF Bray
Oct 1, 2019·Endocrinology and Metabolism·Jierui LiuJun Liang