Mediator-induced changes in macromolecular permeability in the rat mesenteric microcirculation

Microvascular Research
V Laux, D Seiffge

Abstract

An intravital fluorescence microscopic method for measurement of changes in macromolecular permeability has been established in the mesenterial microcirculation of the rat. After exteriorization of the fat-free distal part of the ileal mesentery, a 1-hr period of stabilization was followed by the injection of FITC-labeled macromolecules. Five minutes later, histamine, leukotriene B4, or leukotriene C4 was topically applied to the tissue by means of a micromanipulator. Areas of 1 mm2 were videotaped with a SIT camera. The fluorescence intensity of these areas was measured by an analogous video image processing system and displayed as gray value histograms. The shift of the frequency of gray levels from lower to upper regions could be attributed to an increase in light intensity in the mesentery, indicating an increase in vessel wall permeability. The sites of action of histamine and leukotriene C4 were very similar. Both mediators affected mainly the larger collecting venules. In contrast, leukotriene B4 exerted its effect at postcapillary venules. Moreover, leukotriene B4-induced extravasation was inhibited by superoxide dismutase, suggesting an involvement of oxygen radicals. The studies with histamine alone and with H1- and H...Continue Reading

Citations

Feb 5, 2011·BMC Immunology·Muthuvel ArumugamSimon P Hogan
Jan 11, 2011·Technology in Cancer Research & Treatment·N FayeO Clement
Jun 7, 2002·Australasian Physical & Engineering Sciences in Medicine·H ChenJ L Hu
Jul 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·D SeiffgeR Schleyerbach
Feb 25, 2003·Journal of Applied Physiology·Alfred J CasillanJohn G Wood
Feb 7, 2003·American Journal of Physiology. Gastrointestinal and Liver Physiology·C E King-VanVlackP H MacDonald

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