Mediator subunit MED31 is required for radial patterning of Arabidopsis roots

Proceedings of the National Academy of Sciences of the United States of America
Xiaoyue ZhangChuanyou Li

Abstract

Stem cell specification in multicellular organisms relies on the precise spatiotemporal control of RNA polymerase II (Pol II)-dependent gene transcription, in which the evolutionarily conserved Mediator coactivator complex plays an essential role. In Arabidopsis thaliana, SHORTROOT (SHR) and SCARECROW (SCR) orchestrate a transcriptional program that determines the fate and asymmetrical divisions of stem cells generating the root ground tissue. The mechanism by which SHR/SCR relays context-specific regulatory signals to the Pol II general transcription machinery is unknown. Here, we report the role of Mediator in controlling the spatiotemporal transcriptional output of SHR/SCR during asymmetrical division of stem cells and ground tissue patterning. The Mediator subunit MED31 interacted with SCR but not SHR. Reduction of MED31 disrupted the spatiotemporal activation of CYCLIND6;1 (CYCD6;1), leading to defective asymmetrical division of stem cells generating ground tissue. MED31 was recruited to the promoter of CYCD6;1 in an SCR-dependent manner. MED31 was involved in the formation of a dynamic MED31/SCR/SHR ternary complex through the interface protein SCR. We demonstrate that the relative protein abundance of MED31 and SHR in di...Continue Reading

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Citations

Sep 1, 2020·The EMBO Journal·Bénédicte Desvoyes, Crisanto Gutierrez
Jan 4, 2019·Plants·Riccardo Di MambroRaffaele Dello Ioio
Dec 1, 2020·The New Phytologist·Naohiko OhamaNam-Hai Chua
Jun 27, 2021·Journal of Experimental Botany·Renan Pardal, Renze Heidstra
Sep 10, 2021·The Plant Cell·Robert Sablowski, Crisanto Gutierrez

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Methods Mentioned

BETA
two-hybrid
pull-down
Y2H
Co-IP
immunoprecipitation
PCR
ChIP
pull down
pulled
three-hybrid

Software Mentioned

SHORTROOT

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