Mediators of arachidonic acid-induced contractions of indomethacin-treated guinea-pig airways: leukotrienes C4 and D4

British Journal of Pharmacology
J F Burka, M H Saad


Arachidonic acid (AA) (66 microM) induced contractions of indomethacin-treated (8.4 microM) guinea-pig tracheal and lung parenchymal preparations. Mepacrine (210 microM) treatment did not affect the magnitude of contraction induced by AA. Normal and ovalbumin-sensitized tissues responded identically to AA, and released equivalent amounts of the contractile mediators. Nordihydroguaiaretic acid (100 microM) markedly reduced release of the contractile mediators and reduced AA-induced contractions of the airways. The mediators of AA-induced, calcium ionophore A23187-induced, and antigen-induced contraction of the trachea and lung parenchyma were purified and identified by reverse-phase high performance liquid chromatography to be leukotrienes C4 and D4, being present in an approximate ratio of 20:1. Mepacrine-treated trachea exhibited a smaller contractile response to stimuli (A23187 for normal tissues and ovalbumin for sensitized tissues). Addition of exogenous AA (66 microM) increased the magnitude of contraction, although not to the level observed on tissues not treated with mepacrine. There was no observable effect of AA on the response of mepacrine-treated parenchyma to the ionophore or antigen. It was concluded that (a) immun...Continue Reading


May 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P Borgeat, B Samuelsson
Dec 11, 1974·Biochemical and Biophysical Research Communications·M Hamberg, B Samuelsson
Sep 4, 1980·Nature·B A Jakschik, L H Lee
Jul 4, 1981·Lancet·M C HolroydeE V Elliott
Dec 1, 1981·The Journal of Clinical Investigation·J M Mencia-HuertaJ Benveniste
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H R MorrisJ R Tippins
May 1, 1983·Prostaglandins·M H SaadJ F Burka
Apr 1, 1980·Prostaglandins·J F Burka, N A Paterson
Jul 1, 1980·Allergy·B SamuelssonP Borgeat
Mar 12, 1982·European Journal of Pharmacology·S A Saeed, M D Mitchell
May 1, 1982·Canadian Journal of Physiology and Pharmacology·T R JonesE E Daniel
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J M DrazenE J Corey
Oct 1, 1982·The Journal of Clinical Investigation·D W MacGlashanL M Lichtenstein
Jun 1, 1965·The Journal of Pharmacy and Pharmacology·J W CONSTANTINE

❮ Previous
Next ❯


Jul 1, 1988·Agents and Actions·U Achterrath-TuckermannB A Peskar
Jun 1, 1985·Journal of Autonomic Pharmacology·G Feuerstein
Nov 26, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Takeshi KatayamaKazuhiro Kohama
Apr 1, 1985·British Journal of Pharmacology·A DetsouliB B Vargaftig
Sep 1, 1985·Annales De L'Institut Pasteur. Immunology·B B Vargaftig

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.