Medically Relevant Acinetobacter Species Require a Type II Secretion System and Specific Membrane-Associated Chaperones for the Export of Multiple Substrates and Full Virulence

PLoS Pathogens
Christian M HardingMario F Feldman

Abstract

Acinetobacter baumannii, A. nosocomialis, and A. pittii have recently emerged as opportunistic human pathogens capable of causing severe human disease; however, the molecular mechanisms employed by Acinetobacter to cause disease remain poorly understood. Many pathogenic members of the genus Acinetobacter contain genes predicted to encode proteins required for the biogenesis of a type II secretion system (T2SS), which have been shown to mediate virulence in many Gram-negative organisms. Here we demonstrate that Acinetobacter nosocomialis strain M2 produces a functional T2SS, which is required for full virulence in both the Galleria mellonella and murine pulmonary infection models. Importantly, this is the first bona fide secretion system shown to be required for virulence in Acinetobacter. Using bioinformatics, proteomics, and mutational analyses, we show that Acinetobacter employs its T2SS to export multiple substrates, including the lipases LipA and LipH as well as the protease CpaA. Furthermore, the Acinetobacter T2SS, which is found scattered amongst five distinct loci, does not contain a dedicated pseudopilin peptidase, but instead relies on the type IV prepilin peptidase, reinforcing the common ancestry of these two system...Continue Reading

References

Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D N Nunn, S Lory
Aug 1, 1992·Archives of Biochemistry and Biophysics·M Chihara-SiomiJ Oda
Aug 1, 1993·Molecular Microbiology·L G FrenkenC T Verrips
Jul 8, 1999·Molecular & General Genetics : MGG·M El KhattabiJ Tommassen
Jun 22, 2000·The Journal of Biological Chemistry·M El KhattabiJ Tommassen
May 12, 2001·Infection and Immunity·M Sandkvist
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Marija TauschekRoy M Robins-Browne
Jan 10, 2003·Nucleic Acids Research·Markus Fischer, Jürgen Pleiss
Mar 7, 2003·FEMS Microbiology Letters·Mario F Feldman, Guy R Cornelis
Sep 1, 2005·The Journal of Biological Chemistry·Mohamed ChamiNicolas Bayan
Mar 7, 2006·Nature Structural & Molecular Biology·Kris PauwelsPatrick Van Gelder
Dec 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sruti DebRoyNicholas P Cianciotto
Sep 25, 2007·Journal of Bacteriology·Aleksandra E SikoraMaria Sandkvist
Nov 17, 2007·Nature Reviews. Microbiology·Lenie DijkshoornHarald Seifert
Apr 11, 2008·Molecular Microbiology·Vladimir Pelicic
Apr 1, 2009·Antimicrobial Agents and Chemotherapy·Anton Y PelegEleftherios Mylonakis
Jul 25, 2009·Applied Microbiology and Biotechnology·Bhawna Madan, Prashant Mishra
Sep 21, 2010·Nature Structural & Molecular Biology·Steve L ReichowTamir Gonen
Nov 27, 2010·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Mar 10, 2011·The Journal of Biological Chemistry·Aleksandra E SikoraMaria Sandkvist
Apr 3, 2012·Nature Reviews. Microbiology·Konstantin V KorotkovWim G J Hol
Apr 19, 2012·Biology Direct·Grzegorz M BoratynThomas L Madden
May 10, 2012·FEMS Microbiology Reviews·Michael J McConnellJerónimo Pachón

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Citations

Apr 5, 2017·The Journal of Biological Chemistry·Christian M HardingMario F Feldman
Mar 22, 2018·Emerging Microbes & Infections·Noha M Elhosseiny, Ahmed S Attia
Mar 8, 2017·Infection and Immunity·Nicholas P Cianciotto, Richard C White
Dec 30, 2015·Journal of Bacteriology·Brent S WeberMario F Feldman
Dec 16, 2016·Clinical Microbiology Reviews·Darren WongBrad Spellberg
Dec 19, 2017·Nature Reviews. Microbiology·Christian M HardingMario F Feldman
Jun 18, 2019·Annual Review of Microbiology·Edward GeisingerRalph R Isberg
May 10, 2020·Biomolecules·Maria Soledad RamirezMarcelo E Tolmasky
Oct 30, 2016·The Journal of Biological Chemistry·Kurt H PiepenbrinkEric J Sundberg
Aug 18, 2020·Applied and Environmental Microbiology·Jian PangQingsheng Qi
Mar 30, 2017·Frontiers in Cellular and Infection Microbiology·Chang-Ro LeeSang Hee Lee
Nov 2, 2019·Microbiology Resource Announcements·Bailey PehdeMichael Carruthers
Aug 6, 2019·Frontiers in Microbiology·Faye C MorrisAnton Y Peleg
May 10, 2017·Molecular Microbiology·Jenny-Lee ThomassinOlivera Francetic
Dec 8, 2020·Molecular Microbiology·Souvik NaskarHarry H Low
Feb 23, 2021·Microbiological Research·Hing Jian MeaEng Hwa Wong
Apr 4, 2021·Pathogens·Meysam SarsharCecilia Ambrosi
Apr 27, 2021·Computational and Structural Biotechnology Journal·Xinjie HuiYejun Wang
Apr 29, 2021·The Biochemical Journal·D Judy ShonStacy A Malaker
Feb 16, 2019·EcoSal Plus·Konstantin V Korotkov, Maria Sandkvist
Jul 22, 2021·Critical Reviews in Microbiology·María Marcela CameranesiGuillermo Daniel Repizo

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Methods Mentioned

BETA
electrophoresis
PCR
lipase assay

Software Mentioned

SameSpots
Basic Local Alignment Search Tool ( BLAST )
Mascot
DELTA
TMHMM
GraphPad Prism
SignalP
BLASTp
DELTA BLASTp

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