PMID: 3756134Jul 29, 1986Paper

Medium-chain acyl coenzyme A dehydrogenase from pig kidney has intrinsic enoyl coenzyme A hydratase activity

Biochemistry
S M LauC Thorpe

Abstract

The flavoprotein medium-chain acyl coenzyme A (acyl-CoA) dehydrogenase from pig kidney exhibits an intrinsic hydratase activity toward crotonyl-CoA yielding L-3-hydroxybutyryl-CoA. The maximal turnover number of about 0.5 min-1 is 500-1000-fold slower than the dehydrogenation of butyryl-CoA using electron-transferring flavoprotein as terminal acceptor. trans-2-Octenoyl- and trans-2-hexadecenoyl-CoA are not hydrated significantly. Hydration is not due to contamination with the short-chain enoyl-CoA hydratase crotonase. Several lines of evidence suggest that hydration and dehydrogenation reactions probably utilize the same active site. These two activities are coordinately inhibited by 2-octynoyl-CoA and (methylenecyclopropyl)acetyl-CoA [whose targets are the protein and flavin adenine dinucleotide (FAD) moieties of the dehydrogenase, respectively]. The hydration of crotonyl-CoA is severely inhibited by octanoyl-CoA, a good substrate of the dehydrogenase. The apoenzyme is inactive as a hydratase but recovers activity on the addition of FAD. Compared with the hydratase activity of the native enzyme, the 8-fluoro-FAD enzyme exhibits a roughly 2-fold increased activity, whereas the 5-deaza-FAD dehydrogenase is only 20% as active. A ...Continue Reading

References

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Citations

Jul 20, 2002·Archives of Biochemistry and Biophysics·Teresa R LammMarian T Stankovich
Apr 21, 1999·Biochimica Et Biophysica Acta·A PreusserH P Kleber
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J J KimR Paschke
Jun 1, 1989·Archives of Biochemistry and Biophysics·J Z Zhou, C Thorpe
Feb 1, 1992·Archives of Biochemistry and Biophysics·T C Lehman, C Thorpe
Nov 13, 2012·Molecular Genetics and Metabolism·Kaitlyn KormanikAl-Walid Mohsen
May 5, 1987·Biochemistry·M T Stankovich, S Soltysik
Jan 24, 2019·Journal of Bacteriology·Pascal BazireAlain Perret

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