Medium-chain-length polyhydroxyalkanoates synthesis by Pseudomonas putida KT2440 relA/spoT mutant: bioprocess characterization and transcriptome analysis

AMB Express
Justyna Mozejko-CiesielskaSlawomir Ciesielski

Abstract

Pseudomonas putida KT2440 is a model bacteria used commonly for medium-chain-length polyhydroxyalkanoates (mcl-PHAs) production using various substrates. However, despite many studies conducted on P. putida KT2440 strain, the molecular mechanisms of leading to mcl-PHAs synthesis in reaction to environmental stimuli are still not clear. The rearrangement of the metabolism in response to environmental stress could be controlled by stringent response that modulates the transcription of many genes in order to promote survival under nutritional deprivation conditions. Therefore, in this work we investigated the relation between mcl-PHAs synthesis and stringent response. For this study, a relA/spoT mutant of P. putida KT2440, unable to induce the stringent response, was used. Additionally, the transcriptome of this mutant was analyzed using RNA-seq in order to examine rearrangements of the metabolism during cultivation. The results show that the relA/spoT mutant of P. putida KT2440 is able to accumulate mcl-PHAs in both optimal and nitrogen limiting conditions. Nitrogen starvation did not change the efficiency of mcl-PHAs synthesis in this mutant. The transition from exponential growth to stationary phase caused significant upregulat...Continue Reading

References

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Citations

May 8, 2018·Journal of Industrial Microbiology & Biotechnology·Justyna Mozejko-CiesielskaSlawomir Ciesielski
Apr 9, 2020·Microbial Biotechnology·Andreas AnkenbauerRalf Takors
Mar 14, 2019·Journal of Industrial Microbiology & Biotechnology·Songyuan YangXiaoqiang Jia
Apr 23, 2020·Frontiers in Bioengineering and Biotechnology·Luz YañezFelipe Scott
May 20, 2020·Frontiers in Bioengineering and Biotechnology·Claudia Velázquez-SánchezDaniel Segura
Jun 12, 2020·Frontiers in Bioengineering and Biotechnology·Karina HobmeierKatharina Pflüger-Grau
Jan 7, 2021·Applied Biochemistry and Biotechnology·Siriorn BoonyawanichNipon Pisutpaisal
Dec 31, 2020·International Journal of Molecular Sciences·Dorota DabrowskaSlawomir Ciesielski
Aug 28, 2021·Life·Alexandra PeregrinaCecília M Arraiano
Sep 3, 2021·Microbial Biotechnology·Ruchira MitraJing Han

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Datasets Mentioned

BETA
PRJNA374570

Methods Mentioned

BETA
PCR
electrophoresis
RNAseq
reverse transcription PCR
RNA-seq
environmental stress

Software Mentioned

DESeq
FASTX Tool Kit
SAM
Bowtie
R
STRING

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